人間RAP30/74およびEscherichia coli sigma 70における関連するRNAポリメラーゼ結合領域

S McCracken¹, J Greenblatt

¹Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada.

Science (New York, N.Y.)

|August 23, 1991

まとめ

RAP30/74のような一般的な転写始動因子は,RNAポリメラーゼIIと結合する. RAP30サブユニット

科学分野:

分子生物学は分子生物学である.
バイオケミストリーバイオケミストリー
遺伝学遺伝学とは

背景:

RAP30/74はヘテロメアの一般転写開始因子である.
哺乳類RNAポリメラーゼIIと相互作用する.
RAP30サブユニットは,E. coli sigma 70との配列類似性を共有しています.

研究の目的:

RAP30とE. coli sigma 70の機能的関係を調査する.
RNAポリメラーゼ結合領域が機能的に関連しているかどうかを判断する.

主な方法:

セルリン残留からの保護を評価するためのインビトロリン酸化測定法.
人間のRAP30/74とE. coliRNAポリメラーゼを用いた結合分析.
E. coliシグマ70を用いた移位測定法

主要な成果:

哺乳類RNAポリメラーゼIIは,RAP30シグマ70に関連する領域のセリン残基をリン酸化から保護した.
人間のRAP30/74は,E. coliのRNAポリメラーゼと結合する.
E. coli sigma 70は,E. coli RNAポリメラーゼからヒトRAP30/74を異動させました.

結論:

RAP30とシグマ70は,機能的に関連するRNAポリメラーゼ結合ドメインを有する.
これは,種間の転写開始における保存されたメカニズムを示唆しています.

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