サイクリンAとアデノウイルスE1Aに関連したp33キナーゼをコードするヒトcdk2遺伝子の分離
L H Tsai1, E Harlow, M Meyerson
1Massachusetts General Hospital Cancer Center, Charlestown 02129.
Nature
|September 12, 1991
まとめ
研究者らは,サイクリンA関連タンパク質p33をcdk2遺伝子の産物として特定した. このサイクリン依存キナーゼ2 (cdk2) 複合体とサイクリンAは,脊椎動物の細胞循環の調節にユニークな役割を果たしています.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- バイオケミストリー バイオケミストリー
背景:
- サイクリンは,細胞サイクル進行を制御するキナーゼと複合体を形成する規制タンパク質です.
- p34cdc2/cyclin B複合体は,G2からM段階への移行に不可欠である.
- サイクリンAのような他のサイクリン複合体の機能は,ほとんど不明のままである.
研究 の 目的:
- サイクリンA関連タンパク質p33.3をコードする遺伝子を特定する.
- 新型cdc2関連遺伝子の特徴を明らかにするために,cdk2.
- 細胞循環調節におけるp33cdk2/cyclin A複合体の役割を明らかにする.
主な方法:
- 新しいヒトのcdc2に関連する遺伝子のクローニング.
- 免疫化学的特徴と部分タンパク質分解のマッピング.
- キナーゼ活性を評価するためにp33cdk2タンパク質の免疫プレシピテーション.
主要な成果:
- 遺伝子cdk2が特定され,p34cdc2.2と有意な配列同一性を共有しました.
- cdk2遺伝子製品は,サイクリンA関連タンパク質p33.3として確認されました.
- p33cdk2はインビトロでタンパク質キナーゼ活性を示した.
結論:
- cdk2遺伝子の産物は,サイクリンAに関連したp33.3です.
- p33cdk2/cyclin A複合体は,細胞循環調節に関与している.
- ウイルスのE1Aオンコタンパク質は,p33cdk2/cyclin A複合体と相互作用する.
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