転写因子E2によるBPV-1複製のインビトロ活性化

L Yang¹, R Li, I J Mohr

¹Department of Molecular and Cell Biology, University of California, Berkeley 94720.

Nature

|October 17, 1991

まとめ

ウイルスE1およびE2タンパク質と細胞抽出物は,パピローマウイルスDNA複製を可能にします. E2タンパク質は,原点へのE1結合を強化し,DNA複製を開始し,転写因子を示唆します.

科学分野:

分子生物学は分子生物学である.
ウイルス学ウイルス学ウイルス学
細胞生物学細胞生物学

背景:

パピローマウイルスDNA複製は,ウイルスの伝播に不可欠です.
複製の開始におけるウイルスタンパク質E1とE2の役割は調査中です.
ユカリオットDNAの複製メカニズムを理解することは,生物学の根本的な問題である.

研究の目的:

パピローマウイルスDNA複製の要求を in vitroで調査する.
複製の開始におけるウイルスE1およびE2タンパク質の特定の機能を決定する.
ウイルスのDNA複製における細胞因子と転写因子の潜在的な関与を探求する.

主な方法:

感染していないネズミの細胞から溶解可能な抽出物を使用しています.
純化されたウイルスE1およびE2タンパク質を添加したエキス.
外因的に追加されたパピローマウイルスDNAの複製を評価する.

主要な成果:

E1およびE2タンパク質を含む溶解性の細胞抽出物は,パピローマウイルスDNA複製をサポートしました.
E2トランザクティベータは,E1タンパク質の複製の起源への結合を刺激することが示されました.
E1結合に対するE2の作用は,DNA複製の活性化につながった.

結論:

ウイルスのE1およびE2タンパク質は,細胞因子の存在においてパピローマウイルスDNA複製をサポートするのに十分である.
E2タンパク質は,E1ヘリカーゼをウイルス発生源に誘導する上で重要な役割を果たします.
これらの発見は,転写因子が複製開始複合体を形成することによって,真核細胞DNA複製の開始に直接参加するモデルを支持する.

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