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Updated: Jul 9, 2026

In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
08:55

In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

Published on: March 26, 2012

メチル化されたmRNAの5'-端末構造は,膀性口炎ウイルスによってインビトロ合成される

G Abraham, D P Rhodes, A K Banerjee

    Cell
    |May 1, 1975
    PubMed
    まとめ

    膀性口腔炎ウイルスのRNAポリメラーゼは,ユニークなmRNAキャップ構造を作り出します. この構造m7G(5')ppp(5')A-m-pは,ウイルスのmRNAの機能と安定性にとって不可欠である.

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    Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV14:40

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    Published on: March 5, 2022

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    pre-mRNA Processing02:01

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    In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
    Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
    Pre-mRNA Processing02:01

    Pre-mRNA Processing

    In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
    Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
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    Pre-mRNA Processing: Modification of pre-mRNA Ends

    In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
    Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
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