関連する実験動画

Updated: Jul 19, 2026

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
11:51

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Published on: April 27, 2018

緑色光タンパク質における染色体生物生成の反応進行

Liping Zhang1, Hetal N Patel, Jason W Lappe

  • 1Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287-1604, USA.

Journal of the American Chemical Society
|April 6, 2006
PubMed
まとめ

緑色光タンパク質 (GFP) の成熟には,タンパク質の折りたたみ,サイクル化,酸化を含む3段階のプロセスが含まれています. 酸化水素は,光が現れる前に生成され,分子酸素は中間構造を安定させる可能性がある.

さらに関連する動画

Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection07:25

Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection

Published on: May 24, 2018

Production of IgG Fusion Proteins Transiently Expressed in Nicotiana benthamiana13:29

Production of IgG Fusion Proteins Transiently Expressed in Nicotiana benthamiana

Published on: January 16, 2021

関連する概念動画

Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Photoreceptors and Visual Pathways01:22

Photoreceptors and Visual Pathways

At the molecular level, visual signals trigger transformations in photopigment molecules, resulting in changes in the photoreceptor cell's membrane potential. The photon's energy level is denoted by its wavelength, with each specific wavelength of visible light associated with a distinct color. The spectral range of visible light, classified as electromagnetic radiation, spans from 380 to 720 nm. Electromagnetic radiation wavelengths exceeding 720 nm fall under the infrared category, whereas...