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陽子結合電子伝達は,シトクロームc酸化酵素の陽子ポンプを駆動する

Ilya Belevich¹, Michael I Verkhovsky, Mårten Wikström

¹Helsinki Bioenergetics Group, Institute of Biotechnology, University of Helsinki, FIN-00014 University of Helsinki, Helsinki, Finland.

|April 7, 2006

まとめ

細胞呼吸における電子の移転は,シトクロームc酸化酵素によるプロトンポンプと結びついています. この研究では,ヘムアから酸素還元部位への電子の移転が,内部陽子移転を通じて陽子ポンプメカニズムを開始することを明らかにしました.

科学分野:

バイオケミストリーバイオケミストリー
分子生物学は分子生物学である.
バイオエネルギー学バイオエネルギー学

背景:

細胞呼吸は,電子の移転と陽子の転位を組み合わせて,エネルギー伝導を行う.
電気化学的なプロトン・グラデーションは,ATP合成のような細胞反応に力を与えます.
サイトクロームc酸化酵素は,酸素の減少と呼吸連鎖における陽子ポンプに不可欠です.

研究の目的:

サイトクロームc酸化酵素による陽子ポンプの仕組みを解明する.
内部電子移転と陽子の転位の間の結合を調査する.
陽子ポンプの開始におけるヘムアとO2還元部位の役割を明らかにする.

主な方法:

電気電荷の移動をリアルタイムで監視する.
サイトクロームc酸化酵素内の電子伝達の同時観測.
陽子の吸収と放出の運動分析.

主要な成果:

ヘムアからO2還元部位への電子移動は,陽子ポンプのトリガーとして特定されました.
この電子移転は,内部のベクトル型陽子移転と運動的に結びついています.
陽子ポンプのメカニズムは,水面からの陽子吸収/放出の前に開始されます.

結論:

ヘムAからO2還元部位への内部電子伝達は,直接,シトクロームc酸化酵素陽子ポンプを駆動する.
陽子ポンプは,内部電子伝送と機械的に結合され,以前の運動的示唆を否定する.
この発見は,生物学的エネルギー伝導の重要なステップを明確にします.

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