関連する実験動画
Updated: May 21, 2026

09:41
Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
Published on: June 18, 2014
Wntリガンドの分泌には,保存されたトランスメブランタンパク質であるEviが必要です
Kerstin Bartscherer1, Nadège Pelte, Dierk Ingelfinger
1German Cancer Research Center, Boveri-Group Signaling and Functional Genomics, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany.
Cell
|May 9, 2006
まとめ
Evenness interrupted (Evi/Wls) は,Wntの分泌に不可欠なトランスメブランタンパク質である. その枯渇はWntシグナル伝達を妨害し,これらの重要なシグナル伝達分子を放出する役割を強調します.
科学分野:
- 細胞生物学 細胞生物学
- 発達生物学 発達生物学とは
- 分子遺伝学 分子遺伝学
背景:
- Wnt信号伝達経路は,発達と疾患における重要な生物学的プロセスを調節する.
- Wntタンパク質は,短距離と長距離で作用するシグナル分子を分泌する.
- Wntタンパク質の放出と分泌因子のメカニズムは,依然としてほとんど不明です.
研究 の 目的:
- Wnt.のドロソフィラ同型であるWingless (Wg) の分泌に関与するトランスメブランタンパク質を特定する.
- Wntの分泌とシグナル伝達におけるEvenness interrupted (Evi/Wls) の役割を明らかにする.
主な方法:
- ドロソフィラWgシグナル伝達におけるトランスメブランタンパク質のRNA干渉 (RNAi) スクリーニング.
- エビミュータントにおける発達パターンの欠陥の分析.
- エピスタシス実験とクローン分析.
- 保存された機能を評価するために,ヒトの細胞におけるヒトWlsホモログの枯渇.
主要な成果:
- 保存された多通路トランスメブランタンパク質,Evenness interrupted (Evi/Wls) が特定されました.
- エビ・ミュータントは,発達パターンの欠陥とWg標的遺伝子発現の失敗を示している.
- 人間のWlsの枯渇は,ヒト細胞のWntシグナル伝達を妨害し,進化的保存を確認します.
- Evi/Wlsは,Wgを生成する細胞内で機能し,変異した細胞でWgを保持します.
結論:
- Evi/Wlsは,Wg/Wntタンパク質の分泌に不可欠である.
- Evi/Wlsは,Wntの分泌に特異的に必要な遺伝子ファミリーの最初の特定されたメンバーです.
- Evi/Wlsの機能を理解することで,Wntの分泌メカニズムが発達や病気に不可欠なことを理解できます.
関連する概念動画
Canonical Wnt Signaling Pathway
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Non-Canonical Wnt Signaling Pathways
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
TGF - β Signaling Pathway
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
Canonical Wnt Signaling Pathway
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Non-Canonical Wnt Signaling Pathways
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
Transduction
Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome are...

