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関連する概念動画

Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
Base Excision Repair01:54

Base Excision Repair

One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...

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関連する実験動画

Updated: Jun 25, 2026

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)
13:06

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)

Published on: September 24, 2015

DNA損傷応答経路をマッピングするためのシステムアプローチ.

Christopher T Workman1, H Craig Mak, Scott McCuine

  • 1University of California San Diego, La Jolla, CA 92093, USA.

Science (New York, N.Y.)
|May 20, 2006
PubMed
まとめ
この要約は機械生成です。

DNAの損傷に反応できない細胞は,突然変異を引き起こす可能性があります. この研究では,酵母におけるこの反応を制御する転写ネットワークをマッピングし,DNA損傷シグナル伝達に関与する重要な相互作用と経路を明らかにしました.

さらに関連する動画

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

関連する実験動画

Last Updated: Jun 25, 2026

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)
13:06

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)

Published on: September 24, 2015

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは
  • システム生物学 システム生物学

背景:

  • DNAの損傷に対する細胞の反応は,突然変異の予防と環境毒性の軽減に不可欠です.
  • DNA損傷反応を制御する転写ネットワークを理解することは,細胞防衛機構を理解するために不可欠です.

研究 の 目的:

  • 酵母におけるDNA損傷反応を制御する全ゲノム規模の転写ネットワークをマッピングする.
  • メチルメタネスルフォネート (MMS) に対する反応に関与する転写因子 (TF) -標的相互作用と結合モチーフを特定する.
  • DNA損傷後のシグナル伝達,転写,フェノタイプを統合した因果経路モデルを構築する.

主な方法:

  • 30のダメージに関連する転写因子 (TF) の全ゲノムにわたる結合位置は,メチルメタネスルフォネート (MMS) に曝露された酵母で測定されました.
  • TF標的の相互作用が特定され,TF欠乏酵母と野生型の標的遺伝子発現の変化を評価することによって機能的検証が行われました.
  • 検証された相互作用は,因果経路モデルを構築するために使用されました.

主要な成果:

  • 合計5272のTF-ターゲット相互作用が特定され,MMS曝露時にプロモーター結合パターンの広範な変化を示しました.
  • 損傷特有の結合モチーフが発見され,DNA損傷応答の規制メカニズムに関する洞察を得ました.
  • 機能的検証は,MMSに対する転写応答にとって重要な多数のTF-ターゲットの相互作用を確認した.

結論:

  • この研究は,酵母におけるDNA損傷反応を制御する複雑な転写ネットワークを明らかにした.
  • 特定されたTF-ターゲットの相互作用と経路は,細胞がDNA損傷後のシグナル伝達,転写,および表型的結果をどのように統合するかの全体的な見方を提供します.
  • これは,分子レベルで突然変異と環境有毒性を理解するための基礎を提供します.