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Updated: Jul 18, 2026

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

エンドヌクレアゼのDNA結合および分裂特異性の計算再設計

Justin Ashworth1, James J Havranek, Carlos M Duarte

  • 1Howard Hughes Medical Institute and Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA. ashwortj@u.washington.edu

Nature
|June 2, 2006
PubMed
まとめ

計算によるタンパク質設計は,I-MsoIエンドヌクレアゼのDNA結合特異性を成功裏に変化させた. 再設計された酵素は,新しい標的の切断が強化され,新しい遺伝子治療ツールへの道を開く.

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