核毛孔結合は,誘導可能な酵母遺伝子の最適な発現レベルを提供します

Angela Taddei¹, Griet Van Houwe, Florence Hediger

¹Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH-4058 Basel, Switzerland.

Nature

|June 9, 2006

まとめ

核遺伝子のポジショニングは遺伝子発現に影響を与える. HXK1遺伝子を核の毛穴に移動させることで,その転写が強化され,核の位置が示されました.

科学分野:

分子生物学は分子生物学である.
細胞生物学細胞生物学
遺伝学遺伝学とは

背景:

核は,特定の核機能を促進するサブコンパートメントに編成されています.
芽生えた酵母菌のサイレントクロマチンの領域は,核包膜に集まって,遺伝子の抑制を促進します.
遺伝子転写は核周辺で観察されており,核毛孔複合体の関連に関連しています.

研究の目的:

遺伝子調節における核定位の役割を調査する.
特定の遺伝子の転写的活性化が,それらの核の局所化に変化をもたらすかどうかを決定する.
遺伝子ロカス位置,核毛孔複合体,遺伝子発現レベルとの関係を理解する.

主な方法:

芽生えた酵母菌の亜テロメア遺伝子HXK1の転写活性化を研究した.
HXK1の局所化に対する異なる活性化経路 (非グルコース炭素源対VP16トランザクティベーター) の効果を分析した.
HXK1の移転における3'未翻訳領域 (UTR) の役割を調査した.
核毛孔とHXK1の関連性を操作し,トランスクリプトレベルを測定した.

主要な成果:

非グルコースの炭素源によるHXK1の転写活性化により,核毛穴への転位を引き起こした.
この移転は,HXK1.1の3' UTRに依存していた.
VP16トランザクティベーターによる活性化は,HXK1を核周辺から遠ざけ,ギャラクトーゼ誘導を阻害した.
核毛孔におけるHXK1の局所化を妨害すると,トランスクリプトレベルが変化し,対抗性のレベルが低下し,結合の促進のレベルが上昇しました.

結論:

核の位置は,最適な遺伝子発現レベルを決定する上で重要な役割を果たします.
遺伝子の局所化,特に核毛孔複合体との関連性は,転写を調節する重要な要因である.
3' UTRは,転写活性化中の遺伝子の調節されたポジショニングに重要である.

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