Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Future of Families: Cardiovascular Health Among Young Adults Cohort Study: Rationale, Key Questions, Study Design, and Participant Characteristics.

Journal of the American Heart Association·2025
Same author

Retraction Notice to: shRNA Knockdown of Bmi-1 Reveals a Critical Role for p21-Rb Pathway in NSC Self-Renewal during Development.

Cell stem cell·2023
Same author

A tissue injury sensing and repair pathway distinct from host pathogen defense.

Cell·2023
Same author

Characteristics of salivary telomere length shortening in preterm infants.

PloS one·2023
Same author

Esrrb Regulates Specific Feed-Forward Loops to Transit From Pluripotency Into Early Stages of Differentiation.

Frontiers in cell and developmental biology·2022
Same author

An Esrrb and Nanog Cell Fate Regulatory Module Controlled by Feed Forward Loop Interactions.

Frontiers in cell and developmental biology·2021

関連する実験動画

Updated: Jun 28, 2026

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

RNA干渉による幹細胞の自己再生を剖析する.

Natalia Ivanova1, Radu Dobrin, Rong Lu

  • 1Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA. nivanova@molbio.princeton.edu

Nature
|June 13, 2006
PubMed
まとめ

マウスの胚性幹細胞の自己更新を制御する重要な遺伝子を,機能喪失スクリーニングを使用して特定しました. これらの4つの遺伝子は,多能性を維持し,細胞の運命を調節する新しい役割を持っています.

さらに関連する動画

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
12:44

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Published on: November 11, 2014

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
06:12

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Published on: March 7, 2022

関連する実験動画

Last Updated: Jun 28, 2026

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
12:44

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Published on: November 11, 2014

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
06:12

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Published on: March 7, 2022

科学分野:

  • 発達生物学 発達生物学について
  • 幹細胞生物学 幹細胞生物学
  • 遺伝学 遺伝学とは

背景:

  • 胚性幹細胞 (ESC) の自己再生は,発達と再生医療にとって極めて重要です.
  • ESCの自己再生の遺伝子調節を理解することは,細胞の運命を制御するために不可欠です.

研究 の 目的:

  • マウスのESC自己再生を制御する新しい遺伝的メカニズムを特定する.
  • 多能性の維持における転写レギュレータの役割を調査する.

主な方法:

  • 遺伝子産物をダウンレギュレーションするために,ショートヘアピンRNA (shRNA) 機能喪失スクリーニングを使用した.
  • 潜在的自己更新機能を持つ転写調節体に焦点を当てています.
  • ダイナミックでグローバルな遺伝子発現分析による統合された遺伝子枯渇.

主要な成果:

  • 枯渇がESCの自己更新に悪影響を及ぼす7つの遺伝子を特定しました.
  • これらの4つの遺伝子の自己更新におけるこれまで認識されていない役割を発見した.
  • 特定された遺伝子の細胞運命を調節する特定の生物学的機能を明らかにした.

結論:

  • ESCの自己更新を制御する遺伝的調節体の複雑なネットワークを明らかにした.
  • 多能性を維持するトランスクリプションのレギュレータの重要性を強調した.
  • ESCにおける細胞運命を決定するメカニズムに関する新しい洞察を提供した.