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関連する概念動画

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...

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関連する実験動画

Updated: Jun 27, 2026

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
10:27

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR

Published on: July 30, 2011

A遺伝子を中心としたC. elegansのタンパク質-DNA相互作用ネットワーク.

Bart Deplancke1, Arnab Mukhopadhyay, Wanyuan Ao

  • 1Program in Gene Function and Expression and Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, 01605, USA.

Cell
|June 17, 2006
PubMed
まとめ
この要約は機械生成です。

この研究では,遺伝子中心の酵母1ハイブリッドアッセイを使用して,C. elegansにおける転写因子 (TF) 相互作用をマッピングしています. これは,高度に接続されたタンパク質-DNA相互作用ネットワークを明らかにし,新しいTFを特定し,遺伝子調節に関する洞察を提供します.

さらに関連する動画

Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes
18:38

Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes

Published on: September 26, 2013

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
08:56

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay

Published on: May 5, 2020

関連する実験動画

Last Updated: Jun 27, 2026

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
10:27

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR

Published on: July 30, 2011

Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes
18:38

Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes

Published on: September 26, 2013

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
08:56

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay

Published on: May 5, 2020

科学分野:

  • 分子生物学は分子生物学である.
  • ゲノミクスゲノミクスとは
  • システム生物学 システム生物学

背景:

  • 転写規制ネットワークは,転写因子 (TF) と標的遺伝子の相互作用を通じて遺伝子発現を制御する.
  • これらの相互作用のマッピングは,単細胞生物と比較して,メタゾーン系では困難です.
  • 既存のTFを中心とした方法は,複雑な多細胞生物にはあまり適していません.

研究 の 目的:

  • 遺伝子中心的アプローチを用いて,C. elegansにおける転写因子-標的遺伝子相互作用を体系的にマッピングする.
  • C. elegansの消化管のためのタンパク質-DNA相互作用 (PDI) ネットワークを構築する.
  • 新しいTFを特定し,メタゾアンの遺伝子調節に関する洞察を得るために.

主な方法:

  • 高通量酵母1ハイブリッド (Y1H) アッセイを使用しました.
  • 遺伝子のプロモーターに焦点を当てた遺伝子中心の戦略を採用しました.
  • 72のC. elegans消化管遺伝子プロモーターと117のタンパク質の相互作用を調査した.

主要な成果:

  • 283のタンパク質-DNA相互作用が特定され,高度に接続されたPDIネットワークを形成しました.
  • このネットワークは,C. elegansの消化器系で発現するTFに富んでいる.
  • 以前は特徴づけられていなかったTFの機能的な注釈を提供し,10つの新しい推定TFを特定しました.
  • 複数のPDIをサポートする in vivo 証拠を生成しました.

結論:

  • 遺伝子中心のアプローチは,メタゾアンの転写規制ネットワークの解剖に強力です.
  • 構築されたPDIネットワークは,システムレベルで異なる遺伝子発現に関する貴重な洞察を提供します.
  • この研究は,C. elegans TFsの機能的な注釈を拡張し,新しい規制構成要素を明らかにします.