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Updated: Jul 15, 2026

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
11:16

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization

Published on: July 11, 2012

移行状態の安定化とアルファ-アミノ炭素酸性のアラニンレースメーゼにおけるアルファ-アミノ炭素酸性

Dan T Major1, Kwangho Nam, Jiali Gao

  • 1Department of Chemistry and Supercomputing Institute, Digital Technology Center, University of Minnesota, Minneapolis, Minnesota 55455, USA. major@chem.umn.edu

Journal of the American Chemical Society
|June 22, 2006
PubMed
まとめ

アラニンラセメーズ (AlaR) は,アラニンラセミゼーションの炭酸性を高めるためにピリドクサル5'-リン酸 (PLP) を使用します. 計算による研究は,ピリジニウムイオン安定化ではなく,溶解効果が鍵であり,AlaRが移行状態を安定させることを明らかにしています.

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Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase11:01

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

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Allosteric Regulation

Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or...
Allosteric Regulation01:08

Allosteric Regulation

Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or...
Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

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Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
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Aldehydes and Ketones with Amines: Imine Formation Mechanism

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