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Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons09:21

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons

2.0K
This protocol describes the cell culturing of human midbrain dopaminergic neurons, followed by immunological staining and the generation of neuronal phenotypic profiles from acquired microscopic high-content images allowing the identification of phenotypic variations due to genetic or chemical...
2.0K
Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression10:54

Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

10.0K
In Parkinson's Disease (PD), Substantia Nigra (SNc) dopaminergic neurons degenerate, leading to motor dysfunction. Here we report a protocol for culturing ventral midbrain neurons from a mouse expressing eGFP driven by a Tyrosine Hydroxylase (TH) promoter sequence, harvesting individual fluorescent neurons from the cultures, and measuring their transcriptome using...
10.0K
Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains08:45

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

14.5K
The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of...
14.5K
Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice09:35

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

9.2K
This protocol describes two different environmental manipulations and a concurrent brain infusion protocol to study environmentally-induced brain changes underlying adaptive behavior and brain repair in adult...
9.2K
Primary Culture of Mouse Dopaminergic Neurons11:58

Primary Culture of Mouse Dopaminergic Neurons

39.1K
Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various...
39.1K
Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

26.4K
Dopamine is distinctly regulated in the midbrain nuclei, which contain the cell bodies and dendrites of the dopamine neurons. Here we describe a dissection and sample-handling approach to maximize results, and thus conclusions and insights, on dopamine regulation in the midbrain nuclei of the substantia nigra (SN) and ventral tegmental area (VTA) in...
26.4K

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関連する実験動画

Updated: Jan 8, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
09:35

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

Published on: January 20, 2015

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南洋の生地化学的境界線は南洋の生地化学的境界線である.

I Marinov1, A Gnanadesikan, J R Toggweiler

  • 1Atmospheric and Oceanic Sciences Program, Princeton University, Princeton, New Jersey 08540, USA. imarinov@mit.edu

Nature
|June 23, 2006
PubMed
まとめ
この要約は機械生成です。

南極海とは 南極海のこと.

さらに関連する動画

Primary Culture of Mouse Dopaminergic Neurons
11:58

Primary Culture of Mouse Dopaminergic Neurons

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Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area
09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Published on: August 10, 2012

26.4K

関連する実験動画

Last Updated: Jan 8, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
09:35

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

Published on: January 20, 2015

9.2K
Primary Culture of Mouse Dopaminergic Neurons
11:58

Primary Culture of Mouse Dopaminergic Neurons

Published on: September 8, 2014

39.1K
Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area
09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

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26.4K

科学分野:

  • 海洋学 海洋学 海洋学
  • バイオジオケミストリー バイオジオケミストリー
  • 気候科学 気候科学

背景:

  • 南大海の栄養素と炭素の循環は,世界の二酸化炭素 (CO2) レベルと生物学的生産に大きな影響を与えます.
  • 以前のモデルでは,高緯度地域が大気中のCO2の調節に不可欠であり,栄養素の利用はCO2レベルと密接に関連していることが示された.

研究 の 目的:

  • 南洋の特定の地域が,空海CO2バランスと生物の輸出生産に異なる影響を及ぼしているかどうかを調査する.
  • 炭素循環と生物の輸出について,南大洋内の異なる地域制御を特定する.

主な方法:

  • 海洋一般循環モデルを用いて,南洋の表面栄養素の減少をシミュレートした.
  • 異なる海洋地域における栄養素の利用可能性の変化に対するモデルの反応を分析した.

主要な成果:

  • 大気中のCO2バランスは,主に南極の深水形成地域の生物ポンプと循環によって調節されます.
  • 世界的な生物の輸出生産は,主に亜南極圏の生物のポンプと循環によって制御されています.

結論:

  • 南大洋の南極と南極下部の地域の間には生地化学的な隔たりが存在します.
  • この分離は,ターゲットを絞った気候変動対策が,炭素循環や生物生産に明確な影響を与える可能性を示唆している.