内皮管は,細胞内真空から in vivo に組み立てられる

Makoto Kamei¹, W Brian Saunders, Kayla J Bayless

¹Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.

Nature

|June 27, 2006

まとめ

脊椎動物における血管ルメンの形成は,内皮細胞の真空の融合によって引き起こされる. この研究は,ゼブラフィッシュにおけるインビボの証拠を提供し,血管の発達における重要なメカニズムを明らかにしています.

科学分野:

発達生物学発達生物学について
細胞生物学細胞生物学
血管生物学血管生物学

背景:

皮質管の形成は,臓器の発達に不可欠である.
血管のルメン形成は循環器系の発達に不可欠ですが,体内では十分に理解されていません.
内皮の真空は,長い間,ルメン形成に役割を果たしていると考えられてきました.

研究の目的:

血管ルメン形成のインビボメカニズムを解明する.
管の組立における内皮の真空の役割を調査する.
in vivo と in vitro の内皮管形成を比較するために.

主な方法:

トランスジェニックゼブラフィッシュの高解像度タイムラップ2フォトンの画像撮影.
3Dコラーゲンマトリックスにおけるヒト内皮細胞のインビトロタイムラップ画像.
細胞内および細胞間真空球動力の分析.

主要な成果:

内皮の真空球形成と融合は,体内における血管光の発達を駆動する.
斑馬魚での観察は,真空管媒介によるチューブ組立に関する in vitro の発見を確認し,拡張します.
インテグリンとcdc42/Rac1のシグナル伝達経路は,真空球形成に関与しています.

結論:

内皮の真空のダイナミクスは,体内での血管ルメンの形成の主なメカニズムです.
この研究は,血管発光の真空球仮説に対する最初の決定的なin vivo証拠を提供します.
この発見は,脊椎動物の循環器系の発達に関する理解を深めるものです.

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