Oct-1ホメオドメインのミトスリン酸化と,Oct-1DNA結合活動の調節
N Segil1, S B Roberts, N Heintz
1Laboratory of Molecular Biology, Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021.
Science (New York, N.Y.)
|December 20, 1991
まとめ
オクタマー-1 (Oct-1) 転写因子のリン酸化は,ミトーシス中のDNA結合を阻害する. このメカニズムは,細胞分裂中に観察された遺伝子転写の一般的な停止を説明する可能性があります.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- 遺伝学 遺伝学とは
背景:
- オクタマー-1 (Oct-1) は,ヒストンH2Bを含む,家政遺伝子を調節する重要な転写因子です.
- 細胞サイクル進行には,転写因子の活性が動的に調節される.
研究 の 目的:
- ミトーシス中のOct-1リン酸化の役割を調査する.
- 細胞サイクル中にOct-1DNA結合が調節されるメカニズムを解明する.
主な方法:
- タンパク質キナーゼAを用いた in vitro リン酸化アッセイ.
- in vivoおよびin vitroのDNA結合アッセイは,Oct-1.0のためのものです.
- リン酸化状態とDNA結合活性との相関分析.
主要な成果:
- Oct-1はミトーシス特有のハイパーホスフォリレーションを受けます.
- Oct-1ホメオドメインの特定のリン酸化部位が特定されました.
- この部位のリン酸化により,Oct-1のDNA結合活性がインビトロおよびインビボの両方で抑制されました.
結論:
- Oct-1のミトーシス固有のリン酸化は,そのDNA結合能力を阻害する.
- この抑制は,ミトーシス中に観察される一般的な転写抑制のための潜在的なメカニズムを提供します.
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