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トウモロコシのパラミューテーションには,RNA依存型RNAポリメラーゼが必要です

Mary Alleman¹, Lyudmila Sidorenko, Karen McGinnis

¹Department of Plant Sciences, 303 Forbes Hall, University of Arizona, Tucson, Arizona 85721, USA.

|July 21, 2006

まとめ

Paramutation1 (mop1) のメディエーターは,トウモロコシのエピジェネティックサイレンシングに不可欠なRNA依存RNAポリメラーゼです. mop1遺伝子は重複RNAレベルを維持し,パラミューテーションのための遺伝性染色体状態を確立します.

科学分野:

エピジェネティクスエピジェネティクス
植物遺伝学植物遺伝学
分子生物学は分子生物学である.

背景:

パラミューテーションは,遺伝性表遺伝的アレルの静止を伴う.
トウモロコシでは,b1ロカスでのパラミューテーションは,タンデムリピートによって媒介されます.
パラミューテーション1 (mop1) 遺伝子の媒介者は,このプロセスに不可欠です.

研究の目的:

mop1遺伝子を特定し,特徴づけるために.
b1場所でのパラミューテーションの分子メカニズムを理解するために.
エピジェネティック情報伝達における mop1 の役割を調査する.

主な方法:

mop1遺伝子の地図ベースのクローン.
mop1の特徴は,RNA依存型RNAポリメラーゼ (RDRP) である.
タンデムリピートのトランスクリプションを研究するための核ランオンアッセイ.

主要な成果:

mop1はクローンされ,siRNA生産に関与するRDRPに類似したRDRPと特定されました.
b1 ローカスでのタンデムリピートが,両方の文字列から転写されます.
siRNAは検出されなかったが,mop1 RDRPは,繰り返しRNAレベルを維持することを提案されている.

結論:

mop1 RDRPは,パラミューテーションの確立と維持に不可欠です.
mop1は,トランス作用サイレンシングのためのリピートRNAの値を維持することによって機能する可能性が高い.
このメカニズムは,パラミューテーションにおける遺伝性クロマチンの状態の変化を支えている.

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