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関連する概念動画

The Nucleosome02:33

The Nucleosome

DNA in a human cell is almost 2m long and it is packed inside a tiny nucleus that is only a few microns in diameter. The level of compaction of DNA inside the nucleus is astonishing. It is organized into several sequentially higher levels of compaction to fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
DNA is wound twice around a protein complex called histone core, that consist of 8 histone proteins. This complex...
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3 variants are also...
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the timing and level of...
The Nucleosome02:33

The Nucleosome

DNA in a human cell is almost 2m long and it is packed inside a tiny nucleus that is only a few microns in diameter. The level of compaction of DNA inside the nucleus is astonishing. It is organized into several sequentially higher levels of compaction to fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
DNA is wound twice around a protein complex called histone core, that consist of 8 histone proteins. This complex...
The Nucleosome01:19

The Nucleosome

Human DNA is almost two meters long. However, it is compressed inside a tiny nucleus measuring only a few microns in diameter. To make this degree of compaction possible, DNA is organized into several sequential levels so that it can fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
The Nucleosome Core Particle01:12

The Nucleosome Core Particle

Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...

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Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA
10:40

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Published on: September 11, 2013

ヌクレオソームの位置づけのためのゲノムコード.

Eran Segal1, Yvonne Fondufe-Mittendorf, Lingyi Chen

  • 1Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel. eran.segal@weizmann.ac.il

Nature
|July 25, 2006
PubMed
まとめ
この要約は機械生成です。

ゲノムには内在のコードが含まれており,それが核細胞の組織を決定し,DNAのアクセシビリティに影響を与えます. このコードは,すべての核細胞の位置の約半分を vivo で説明し,遺伝子調節に影響を与えます.

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Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

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In Situ Nucleosome Assembly for Single-Molecule Correlative Force and Fluorescence Microscopy
05:58

In Situ Nucleosome Assembly for Single-Molecule Correlative Force and Fluorescence Microscopy

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関連する実験動画

Last Updated: May 13, 2026

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA
10:40

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Published on: September 11, 2013

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
06:32

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

Published on: March 9, 2022

In Situ Nucleosome Assembly for Single-Molecule Correlative Force and Fluorescence Microscopy
05:58

In Situ Nucleosome Assembly for Single-Molecule Correlative Force and Fluorescence Microscopy

Published on: September 6, 2024

科学分野:

  • 分子生物学は分子生物学である.
  • ゲノミクスゲノミクスとは
  • エピジェネティクス エピジェネティクス

背景:

  • ユカリオットのゲノムは,DNAを包装し,タンパク質のアクセスを制御するヌクレオソームに編成されています.
  • 核細胞は配列の偏好を示し,鋭く曲がるDNA配列により強く結合します.
  • これらの配列偏好がニュクレオソームの位置づけと遺伝子調節にインビボでの関連性は不明である.

研究 の 目的:

  • DNA配列のインフィーブ・ヌクレオソームの位置づけに対する影響を調査する.
  • DNA配列に基づいてゲノム全体の核細胞組織を予測するモデルを開発する.
  • 内在的な核細胞組織が染色体機能にどのように影響するかを理解する.

主な方法:

  • 酵母から核細胞結合DNA配列を高解像度で分離した.
  • ニュクレオソーム-DNA相互作用をモデル化するための計算アプローチの開発.
  • ニュクレオソーム-DNA相互作用モデルの実験的検証.
  • ゲノム全体のヌクレオソーム組織の予測.

主要な成果:

  • ゲノムは,DNA配列に基づいた内在的な核細胞組織をコードする.
  • この内在的な組織は,in vivoで観察された核細胞の位置の約50%を説明します.
  • 検証された核細胞-DNA相互作用モデルが構築されました.

結論:

  • DNAの配列は本質的に核細胞の組織を in vivo で決定する.
  • 核子の位置付けは,DNAのアクセシビリティに影響を与える重要な規制メカニズムです.
  • この位置付けコードは,転写やクロマチンの改造などの重要な染色体機能に影響を与えます.