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関連する概念動画

Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
NF-kB-dependent Signaling Pathway02:26

NF-kB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

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関連する実験動画

Updated: Jul 9, 2026

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol
08:20

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol

Published on: December 30, 2016

FOXP3は,NFATとの協力を通じて,T細胞機能を制御しています.

Yongqing Wu1, Madhuri Borde, Vigo Heissmeyer

  • 1Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, CO 80309, USA.

Cell
|July 29, 2006
PubMed
まとめ

調節性T細胞 (Tregs) は,免疫反応を抑制するためにユニークなNFAT-FOXP3複合体を使用します. この相互作用により,T細胞の活性化プログラムがスイッチされ,Tレグ抑制機能が機能し,自己免疫糖尿病の治療が可能になります.

さらに関連する動画

In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
08:02

In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction

Published on: January 22, 2020

In Vitro Functional Analysis of Regulatory T cells: Focus On Proliferation And Differentiation
10:21

In Vitro Functional Analysis of Regulatory T cells: Focus On Proliferation And Differentiation

Published on: June 9, 2026

関連する実験動画

Last Updated: Jul 9, 2026

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol
08:20

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol

Published on: December 30, 2016

In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
08:02

In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction

Published on: January 22, 2020

In Vitro Functional Analysis of Regulatory T cells: Focus On Proliferation And Differentiation
10:21

In Vitro Functional Analysis of Regulatory T cells: Focus On Proliferation And Differentiation

Published on: June 9, 2026

科学分野:

  • 免疫学 免疫学とは
  • 分子生物学は分子生物学である.
  • 構造生物学 構造生物学とは

背景:

  • 活性化されたT細胞の核因子 (NFAT) は,T細胞の活性化に不可欠です.
  • NFATは通常,AP-1転写因子と提携してT細胞遺伝子を調節する.
  • 調節性T細胞 (Tregs) は,独特の分子機構を必要とする独特の機能を備えています.

研究 の 目的:

  • 調節性T細胞 (Treg) 機能の基礎にある分子機構を調査する.
  • Tregs.でNFATと協力する転写因子を特定する.
  • NFAT-FOXP3の相互作用の構造的基礎とその機能的結果を解明する.

主な方法:

  • NFAT:FOXP2:DNA複合体の構造を決定するX線結晶学.
  • 構造的な洞察に基づくFOXP3のサイト指向型変異.
  • マウスモデルでの遺伝子発現 (IL2,CTLA4,CD25) と抑制剤活性を測定する機能的測定法.

主要な成果:

  • Tregs.でNFATとFOXP3の間の新しい協力複合体を特定しました.
  • NFATとFOXP3 (FOXP2構造を使用) の間の広範なタンパク質-タンパク質相互作用インターフェースを明らかにしました.
  • NFAT-FOXP3の相互作用を妨害すると,IL2抑制および抑制活性を含むTreg機能が低下することが示されました.

結論:

  • NFATはTregsにおけるFOXP3と連携し,従来のT細胞におけるAP-1との役割とは異なる.
  • このNFAT-FOXP3複合体は,Treg媒介の免疫抑制に不可欠である.
  • NFAT-FOXP3の相互作用をターゲットにすることは,自己免疫疾患に対する潜在的な治療戦略を提供します.