トレポネマ・プリティア (Treponema primitia primitia) のフラゲラモーターのイン・シットゥー構造は,完全に完成している

Gavin E Murphy¹, Jared R Leadbetter, Grant J Jensen

¹Division of Biology, California Institute of Technology, Pasadena, California 91125, USA.

Nature

|August 4, 2006

まとめ

研究者たちは,細菌のフラゲラモーターを視覚化しました.

科学分野:

微生物学微生物学とは
構造生物学構造生物学とは
ナノテクノロジーナノテクノロジー

背景:

バクテリアのフラジェラモーターは,バクテリアの運動性を可能にする複雑なナノマシンです.
その構造はステーターとローターで構成され,ステータのインシット組織と相互作用に関する知識は限られている.
以前の研究は主にローターの部品に焦点を当てていた.

研究の目的:

バクテリアのフラゲラモーターの完全な in situ 構造を決定する.
ステーターの組立,対称性,細胞内の相互作用を明らかにする.
トピック生成と方向変化の構造的基礎を調査する.

主な方法:

電子冷凍撮影は,Treponema primitia.の細胞全体をイメージするために使用されました.
20個の個々のモーター粒子の計算式抽出,並べ替え,平均を計算した.
完全なフラジェラモーターの高解像度 (7nm) 3D再構築が達成されました.

主要な成果:

完全なフラジェラモーターの in situ 構造は 7 nm の解像度で解像しました.
16倍対称性を特徴とするステータアセットは,初めて可視化されました.
ステータは,ローター,Cリング,および新しいPリングのような構造に直接接続することが観察されました.
モーターの大きなサイズは,トルク生成の強化のためのメカニズムを示唆しています.

結論:

この研究は,細菌のフラゲラモーターの完全な構造を初めて in situ に見ることができます.
その結果,ステーターの対称性と他のモーター部品との直接的な接続が明らかになりました.
この結果は,FliGの特定のドメインがCリングの相互作用を含むトルク生成モデルをサポートしています.

さらに関連する動画

09:12

Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System

Published on: March 10, 2020

08:16

Single-Molecule Analysis of Sf9 Purified Superprocessive Kinesin-3 Family Motors

Published on: July 27, 2022

関連する概念動画

01:18

ATP Synthase: Structure

ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...

01:24

Microtubules in Cell Motility

Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...

01:19

The Structure of Intermediate Filaments

The intermediate filaments are one of three widely studied cytoskeletal filaments. They are so named as their diameter (10 nm) is in between that of microfilaments (7 nm) and the microtubules (25 nm). These filaments are highly stable and can remain intact when exposed to high salt concentrations and detergents. These filaments are responsible for providing stability and mechanical support to the cells. They also help in cell adhesion and maintaining tissue integrity.
Intermediate filaments...

01:24

Microtubules in Cell Motility

01:18

Flagella and Motility in Bacteria

Flagella are specialized, thread-like structures that extend from a bacteria's cell envelope. They play a crucial role in motility and chemotaxis. Their structural organization and functioning exemplify sophisticated biological engineering, enabling bacterial survival and adaptability in diverse environments.Structure of the FlagellumA bacterial flagellum consists of three key components: the filament, the hook, and basal body. The filament, a long, helical structure composed of repeating...

01:28

Fimbriae, Pili, and Axial Filaments

Fimbriae and pili are specialized bacterial surface structures that play pivotal roles in adhesion, genetic exchange, and motility. Composed primarily of pilin protein, these hairlike appendages are crucial for bacterial survival and pathogenicity in various environments.Fimbriae: Adhesion and PathogenicityFimbriae are fine, filamentous structures measuring 2–10 nanometers in diameter and are densely distributed on the bacterial cell surface. They facilitate bacterial adhesion to abiotic...

関連する実験動画