活性化された内皮のVCAM-1は,白血球インテグリンVLA-4とVLA-4/フィブロネクチン結合部位とは異なる部位で相互作用する

M J Elices¹, L Osborn, Y Takada

¹Division of Tumor Virology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.

Cell

|February 23, 1990

まとめ

インテグリンVLA-4は,血管細胞結合分子-1 (VCAM-1) の受容体として働き,リンパ球結合を媒介する. この相互作用は,単核白血球を炎症部位に勧誘するのに極めて重要です.

科学分野:

免疫学免疫学とは
細胞生物学細胞生物学
分子医学は分子医学である.

背景:

サイトカインで活性化された内皮細胞は,血管細胞粘着分子-1 (VCAM-1) を発現する.
VCAM-1はリンパ球結合に作用する.
VCAM-1受容体を理解することは,免疫細胞の密輸の鍵です.

研究の目的:

リンパ球におけるVCAM-1の特定の受容体を特定する.
VCAM-1-媒介細胞結合を制御する分子相互作用を解明する.
VLA-4のVCAM-1とフィブロネクチンとの相互作用を区別する.

主な方法:

K-562細胞をVLA-4α4サブユニットcDNAで変異させる.
細胞のVCAM-1とフィブロネクチンへの粘着を評価する.
抑制研究のために,抗VLA-4抗体と溶性フィブロネクチン断片を使用する.

主要な成果:

VLA-4 (Very Late Antigen-4) は,VCAM-1の特定の受容体として特定されました.
K-562細胞のVLA-4発現は,VCAM-1への特異的付着を媒介した.
また,VLA-4は,フィブロネクチンのヘパリンII結合領域 (FN-40) への粘着を媒介した.
VLA-4/VCAM-1およびVLA-4/フィブロネクチン相互作用は,特定の阻害剤によって区別されました.

結論:

VLA-4/VCAM-1リガンド-受容体ペアが特定されました.
この相互作用は,炎症部位への単核白血球の徴募において有意である.
VLA-4は,VCAM-1とフィブロネクチンに固有の結合特性を示しています.

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