トランスジェニックマウスの網膜母細胞腫
J J Windle1, D M Albert, J M O'Brien
1Regulatory Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037.
Nature
|February 15, 1990
まとめ
研究者は,子供の眼がんである網膜芽細胞腫のマウスモデルを作成しました. マウスの網膜に特定のウイルス腫瘍遺伝子を導入すると,ヒトの網膜芽細胞腫と同一の腫瘍が生じ,新しい研究ツールが提供されました.
科学分野:
- 腫瘍学 腫瘍学
- 遺伝学 遺伝学とは
- オフタルモロジック (眼科)
背景:
- 網膜母細胞腫は,網膜母細胞腫遺伝子 (Rb) の変異に関連した子供の眼がんです.
- Rb遺伝子は,腫瘍抑制に不可欠なp105Rbタンパク質をコードする.
- 網膜芽細胞腫の遺伝的基礎を理解することは,がん研究にとって鍵となるものです.
研究 の 目的:
- 網膜芽細胞腫の研究のための新しい動物モデルを開発する.
- 網膜芽細胞腫の発達におけるウイルス性腫瘍遺伝子の役割を調査する.
- ウイルスのオンコタンパク質とRbタンパク質の相互作用を in vivoで探求する.
主な方法:
- シミアンウイルス40 T抗原の眼部特異的発現を持つトランスジェニックマウスの生成.
- 誘導眼腫瘍のヒストロ,超構造,免疫ヒスト化学分析.
- 腫瘍細胞におけるp105RbとT抗原の関連性に関する研究.
主要な成果:
- トランスジェニックのマウスは,ヒトの網膜芽細胞腫に似た遺伝性眼球腫瘍を発症した.
- 腫瘍はヒトの網膜芽細胞腫と同一の組織学,超構造,および免疫ヒスト化学的特徴を示した.
- マウスの腫瘍細胞におけるp105RbとT抗原の間の特定の関連性が見つかりました.
結論:
- Rb結合オンコタンパク質の眼球特異的発現は,網膜芽細胞腫の腫瘍発生を誘導する可能性があります.
- このマウスモデルは,ウイルスオンコタンパク質によるRbの機能不活性化に対するインビボの証拠を提供します.
- 開発されたマウスモデルは,網膜芽細胞腫の病原性および治療戦略の研究に価値があります.
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