GMP-140によって媒介される活性化された内皮への迅速な中性粒子の粘着
J G Geng1, M P Bevilacqua, K L Moore
1St. Francis Medical Research Institute, Oklahoma City, Oklahoma.
Nature
|February 22, 1990
まとめ
顆粒膜タンパク質-140 (GMP-140) は白血球の粘着を媒介し,他のセレクトインと同様に機能します. このタンパク質は,内皮細胞の粘着性を高めることで,中性粒子を炎症部位に誘導する可能性があります.
科学分野:
- 分子生物学は分子生物学である.
- 免疫学 免疫学とは
- 細胞生物学 細胞生物学
背景:
- 粒状膜タンパク質-140 (GMP-140),PADGEMとも呼ばれ,血小板および内皮細胞のグリコタンパク質である.
- GMP-140は構造的にELAM-1とMEL-14に関連しており,細胞結合に関与するセレクトリン遺伝子ファミリーを定義しています.
研究 の 目的:
- 細胞粘着におけるGMP-140の機能的役割を調査する.
- GMP-140と他のセレクトインスとの機能的な類似性を確立する.
主な方法:
- COS細胞をGMP-140補完DNAで変異する.
- 浄化されたGMP-140と活性化された内皮細胞を用いた結合測定法.
- GMP-140.0に対する抗体を用いた抑制研究
主要な成果:
- 人間の中性粒子の細胞とHL-60細胞は,GMP-140.0に特異的に結合する.
- 細胞結合はカルシウムに依存し,活性中性粒子の代謝を必要としない.
- 活性化された内皮細胞は中性粒子を粘着させ,GMP-140抗体によって抑制される相互作用になります.
結論:
- GMP-140は白血球の粘着を媒介し,機能的には他のセレクトインと似ています.
- 活性化された内皮で発現するGMP-140は,中性粒子の炎症部位への募集を促進する可能性があります.
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