Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Mass Spectrometry: Aldehyde and Ketone Fragmentation01:09

Mass Spectrometry: Aldehyde and Ketone Fragmentation

In mass spectrometry, the fragmentation of aliphatic aldehydes and ketones generally occurs through three key mechanisms: α-cleavage, inductive cleavage, and the McLafferty rearrangement.
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Rotating hinge expandable prostheses may have a higher risk of medial tibial plateau deformity compared to constrained hinged expandable prostheses following reconstruction of distal femur in children with primary bone tumors.

European journal of orthopaedic surgery & traumatology : orthopedie traumatologie·2026
Same author

Bioorthogonal chemical labeling of endogenous receptors in the live mouse brain.

Methods in enzymology·2026
Same author

Single-molecule kinetic exploration of functional sub-states in an evolving phosphotriesterase.

Nature communications·2026
Same author

In-house three dimensional-printed cutting guides improve surgical accuracy in children who underwent resection of malignant bone tumours of lower limb and reconstruction with allograft.

International orthopaedics·2026
Same author

A press-fit tibial smooth stem may preserve proximal tibial physeal growth after expandable prosthesis reconstruction following resection of distal femur in children with bone sarcoma.

European journal of orthopaedic surgery & traumatology : orthopedie traumatologie·2026
Same author

Construction of a CO<sub>2</sub>-Fixing Compartment Using a Shape-Transforming DNA Scaffold.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026

関連する実験動画

Updated: Jul 20, 2026

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis
08:46

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

超分子ヒドロゲルを用いた光レクチン配列は,様々なグリココンジュガートの単純な検出とパターンプロファイリングのために使用されます.

Yoichiro Koshi1, Eiji Nakata, Hiroki Yamane

  • 1PRESTO (Synthesis and Control, JST), Department of Synthetic Chemistry and Biological Chemistry, Kyoto University, Kyotodaigaku-Katsura, Nishikyo-ku, Kyoto, 615-8510, Japan.

Journal of the American Chemical Society
|August 10, 2006
PubMed
まとめ

ハイドロゲルマトリックス内の新しい光レクチン配列は,グリコ結合体の高速で高通量分析を可能にします. この方法は,ラベル付けや洗浄なしで敏感なサッカライドの検出を可能にし,複雑な生物サンプル分析を簡素化します.

さらに関連する動画

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

関連する実験動画

Last Updated: Jul 20, 2026

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis
08:46

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

科学分野:

  • バイオケミストリー バイオケミストリー
  • アナリティカル・ケミストリー (Analytical Chemistry) とは
  • マテリアルサイエンス 材料科学

背景:

  • グライココンジュガートは生物学的プロセスにおいて極めて重要であり,効率的な分析方法が必要である.
  • サッカリド分析のための既存の技術は,複雑で時間がかかる可能性があります.

研究 の 目的:

  • 高通量サッカリド検出のための新しい光レクチン配列の開発.
  • レクチンの安定性と機能を強化するために,超分子ヒドロゲルマトリックスを使用します.

主な方法:

  • 超分子ヒドロゲル内の光レクチン配列の構築.
  • 半湿気条件下における光レクチンの非共性不動化.
  • サッカリド検出のための二分子光解消および回復 (BFQR) の適用.

主要な成果:

  • 固定されたレクチンは,ヒドロゲルマトリックス内で分子認識能力を保持した.
  • グルコース,オリゴサッカライド,およびグリコタンパク質を含む様々なサッカライドの光読出が成功しました.
  • 細胞溶解物などの複雑なサンプルを高通量で検知し,プロファイリングすることが実証されています.

結論:

  • 開発されたレクチン配列は,グリココンジュガート分析のための敏感で効率的なプラットフォームを提供します.
  • ハイドロゲルマトリックスはレクチン機能を効果的に保ち,堅固なサッカリド検出を可能にします.
  • この方法は,ターゲットラベルと洗浄のステップの必要性を排除することにより,分析を簡素化します.