まとめ
体外循環と大動脈のクロスクランプを使った冠動脈心臓手術は,左心室の細胞損傷を引き起こしました. これらの変化は,膜の透過性の変化が心臓外科手術の主要な問題であることを示唆しています.
科学分野:
- 心血管外科手術についてです.
- 細胞生物学 細胞生物学
- バイオケミストリー バイオケミストリー
背景:
- 冠動脈の心臓手術には,しばしば心肺バイパスと大動脈のクロスクランプが含まれます.
- これらの手順が心筋に与える細胞の影響を理解することは,患者のアウトカムにとって極めて重要です.
研究 の 目的:
- 左心室における体外循環と大動脈のクロスクランプのヒストロ化学的および超構造的効果を調査する.
- 心肺バイパス手術中の最初の細胞傷害を特定するために.
主な方法:
- 左心室のドリル生検サンプルは,バイパス手術前と後の22人の患者から採取した.
- ヒストロケミカル分析により,酵素活性 (スッキン酸脱水酸化酵素,シトクロム酸化酵素,LDH,フォスフォリラーゼA,ミオシンATPaase) とフォスフォリピドレベルが評価されました.
- 超構造検査により,細胞とミトコンドリアの完全性を評価した.
主要な成果:
- ヒストロケミカル分析では,バイパス後のフォスフォリラーゼAとミオシンATPアゼの活性が低下した,サクシン酸脱水素酵素,シトクロム酸化酵素,LDHの活性が増加したことが明らかになった.
- フリーフォスフォリピドのレベルが上昇し,膜の整合性の変化を示しています.
- 超構造的な発見には,グリコゲン損失,細胞内腫れ,ミトコンドリア損傷 (マトリックス密度の損失,結晶,および破壊) が含まれていた.
結論:
- 観察された左心室の細胞の変化は,イシュケミア-再注射損傷に似ています.
- この発見は,体外循環と大動脈のクロスクランプの主要な結果として,膜の透過性調節の障害があることを示唆している.
関連する概念動画
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