ホモフィルの粘着分子としてのミエリンP0タンパク質の役割
M T Filbin1, F S Walsh, B D Trapp
1Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Nature
|April 26, 1990
まとめ
周辺神経系のミエリン
科学分野:
- 神経科学は神経科学である.
- 細胞生物学 細胞生物学
- バイオケミストリー バイオケミストリー
背景:
- シュヴァン細胞によって形成される外周神経系ミエリンが,神経衝動の急速な伝導を可能にします.
- コンパクトなミエリン構造を維持するメカニズムは不明である.
- P0タンパク質は,外周神経系ミエリンで最も豊富に存在するタンパク質です.
研究 の 目的:
- 周辺神経系のミエリン構造の維持におけるP0タンパク質の役割を調査する.
- P0タンパク質が,ミエリン層を一緒に保つためにホモフィリック相互作用を媒介するという仮説を検証するために.
主な方法:
- 中国ハムスターの卵巣 (CHO) 細胞を感染させ,P0タンパク質を発現させる.
- サスペンション中の細胞粘着と結合を評価する.
- 細胞間の相互作用と膜構造の顕微鏡分析.
主要な成果:
- P0タンパク質を発現するCHO細胞は,サスペンションで大きな塊を形成した.
- P0タンパク質が欠けている細胞は,有意な結合を示さなかった.
- P0発現する細胞は,ペーパー膜の間のデスマソームのような構造を特異的に形成した.
結論:
- P0タンパク質は,同性愛性の粘着分子として作用する.
- P0媒介の粘着は,外周神経系のミエリンがコンパクトな構造を持つことに関与する.
- P0タンパク質が免疫グロブリンスーパーゲンファミリーに含まれていることは,細胞の認識と結合におけるP0タンパク質の役割を支持する.
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