不変連鎖は,MHCクラスIIの分子の免疫学的認識に影響を与える
1Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
Nature
|May 10, 1990
まとめ
インヴァリアントチェーン (II) は,細胞がT細胞に抗原を提示する方法に影響を与えます. Iiがなければ,細胞は変化したMHCクラスII分子を表示し,T細胞の認識と抗体結合に影響を与えます.
科学分野:
- 免疫学 免疫学とは
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
背景:
- 細胞内イベントは,CD4+T細胞によって認識されるMHCクラスIIペプチド複合体の形成に不可欠です.
- インヴァリアントチェーン (Ii) は,MHCIIクラス分子と細胞内に関連した非ポリモルフなグリコタンパク質である.
- 処理された抗原とMHCクラスIIの相互作用を調節するIIの役割は調査中です.
研究 の 目的:
- MHCクラスIIペプチド複合体の形成と表示におけるインヴァリアントチェーン (II) の役割を調査する.
- Iiの存在がMHCクラスIIの分子による抗原処理とプレゼンテーションに影響するかどうかを判断する.
主な方法:
- MHC II クラスII を表す,II を含む,または含まない,トランスフェクトした線維芽細胞の細胞系を生成する.
- これらの細胞ラインの抗原処理とプレゼンテーション能力の分析.
- T細胞アッセイとモノクローナル抗体結合を用いた細胞表面MHCクラスII構成の特徴化.
主要な成果:
- Iiの存在は,細胞の無傷な抗原を処理し,提示する能力に影響しませんでした.
- Ii陰性細胞は,細胞表面でMHCクラスIIの変異した形状を示した.
- Ii陰性細胞におけるこの変化したMHCクラスII構成は,T細胞に対する抗原性ペプチドのプレゼンテーションを増加させた.
- Ii陰性細胞は,いくつかのクラスII特異のモノクローナル抗体の結合が低下したことを示した.
結論:
- インヴァリアントチェーン (II) は,MHCクラスIIの分子の構成を調節する上で重要な役割を果たします.
- Iiは,MHCクラスIIの構造に影響することによって,T細胞に対する抗原性ペプチドのプレゼンテーションに影響します.
- 細胞表面MHCクラスIIの形状は,T細胞の認識と抗体結合に不可欠である.
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