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Updated: Jul 7, 2026

Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System
11:47

Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System

Published on: August 1, 2016

自然の酵素活性部位を,遺伝的にコードされた非自然なアミノ酸で改善する

Jennifer C Jackson1, Sean P Duffy, Kenneth R Hess

  • 1Franklin and Marshall College, Department of Chemistry, P.O. Box 3003, Lancaster, Pennsylvania 17604, USA.

Journal of the American Chemical Society
|August 24, 2006
PubMed
まとめ

不自然なアミノ酸 (UAA) は酵素の活性を大幅に高め,ニトロレドクタゼを本来の状態と比較して30倍以上改善します. このブレークスルーは,酵素工学と基板調節のための強力な新しい方法を提供します.

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A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

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Identifying Amino Acid Overproducers Using Rare-Codon-Rich Markers

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