自然産物のグリコシルトランスフェラーゼ触媒反応の可逆性を利用する
Changsheng Zhang1, Byron R Griffith, Qiang Fu
1Laboratory for Biosynthetic Chemistry, Pharmaceutical Sciences Division, School of Pharmacy, National Cooperative Drug Discovery Group Program, University of Wisconsin (UW)-Madison, 777 Highland Avenue, Madison, WI 53705-2222, USA.
Science (New York, N.Y.)
|September 2, 2006
まとめ
グリコシルトランスファーゼ (GT) は通常片方向性ですが,この研究では,カリケアミシンとバンコマイシン経路からの4つのGTが,可逆反応を触媒化することを示しています. この可逆性は,天然製品の多様性を拡大し,新しいグリコシル化化合物の作成に役立ちます.
科学分野:
- バイオケミストリー バイオケミストリー
- 酵素学 酵素学とは
- 自然製品バイオシンセシス 自然製品バイオシンセシス
背景:
- グリコシルトランスファーゼ (GT) は,複雑な炭水化物を合成する際に重要な酵素である.
- GTは伝統的に,一方的な反応を触媒化する酵素として見られています.
研究 の 目的:
- グリコシルトランスフェラーゼの触媒的可逆性を調査する.
- 多様な天然製品アナログを生成するための可逆GTの潜在能力を探求する.
主な方法:
- カリケアミシンとバンコマイシン経路から精製されたグリコシルトランスフェラーゼを用いた酵素分析.
- 分析技術による反応産物の特徴化.
主要な成果:
- 4つのグリコシルトランスフェラーゼは,糖とアグリコンの交換を可能にする,容易な可逆性触媒を示した.
- カリケアミシンとバンコマイシンの70種類以上の異なるグリコシル化変種が合成されました.
- この研究は,逆転性GT活動の一般的適用性に関する証拠を提供します.
結論:
- グリコシルトランスフェラーゼで触媒化された反応は,逆転可能であり,従来の一方向的な見方に異議を唱えます.
- この可逆性は,新しい核酸糖と多様な天然製品を生成するための強力なツールを提供します.
- リバーシブルGTは,複雑な生物系におけるインビトロ酵素活性のために利用できる.
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