DFTMPは,生物学的試料のほぼ中性pHを評価するためのNMR反応剤である
Michael D Reily1, Lora C Robosky, Matthew L Manning
1Pfizer Global Research and Development, 2800 Plymouth Road, Ann Arbor, Michigan 48105, USA. michael.reily@pfizer.com
Journal of the American Chemical Society
|September 21, 2006
まとめ
新しい反応剤であるディフルオロトリメチルシラニルフォスフォニック酸 (DFTMP) は,NMRを用いて生物学的液体のpHを正確に in situで測定することを可能にします. この多核指標は,複雑なサンプルのpHを正確に測定するための既存の方法よりも利点があります.
科学分野:
- アナリティカル・ケミストリー (Analytical Chemistry) とは
- バイオケミストリー バイオケミストリー
- 核磁共振 (NMR) スペクトロスコピー
背景:
- 生物学的過程を理解するために,正確なin situ pHの決定は極めて重要です.
- 既存のpH指標は,生物サンプルやNMRスペクトルに干渉する可能性があります.
- 複雑な生物流体に対して,信頼性の高い,干渉しないpH指標が必要である.
研究 の 目的:
- 新しいNMR化学シフト標準およびpH指標として,ディフルオロトリメチルシラニルフォスフォン酸 (DFTMP) を導入する.
- 複雑な生物流体におけるin situ pHの測定のためのDFTMPの有用性を実証する.
- 前述のpH感度の高いコンポーネントに比べてDFTMPの優位性を強調する.
主な方法:
- ディフルオロトリメチルシラニルフォスフォン酸 (DFTMP) の合成と特徴付け.
- DFTMPのNMR信号のpH依存性の評価. DFTMPのNMR信号のpH依存性の評価. DFTMPのNMR信号のpH依存性の評価. DFTMPのNMR信号のpH依存性の評価. DFTMPのNMR信号のpH依存性の評価. DFTMPのNMR信号のpH依存性の評価.
- NMR光譜を用いた水溶液および生物流体におけるin situ pH測定のためのDFTMPの応用.
主要な成果:
- DFTMPは,pHに依存するNMRの化学変化を示す.
- 5から8のpH範囲で0.02のRMSEのpH単位で,正確なin situ pH測定を行っています.
- DFTMPは金属結合親和性がなく,内生スペクトル領域の干渉は最小限である.
結論:
- DFTMPは,複雑な生物学的システムにおける正確なin situ pHの測定に役立つ有価な反応剤として機能します.
- 多核能力 (19F,1H,31) と干渉しない性質により,既存の方法よりも優れている.
- DFTMPは,正確でリアルタイムなpHモニタリングを必要とするNMR実験に広く適用できます.
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