関連する実験動画

Updated: Jul 19, 2026

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
09:31

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

Published on: September 26, 2020

TRPケージミニタンパク質の構造と安定性のステレオ電子チューニング

Devan Naduthambi1, Neal J Zondlo

  • 1Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA.

Journal of the American Chemical Society
|September 21, 2006
PubMed
まとめ

研究者はプロリン構成を修正することで,タンパク質の安定性を調整した. プロリンを特定の誘導体に置き換えることで,タンパク質の構造と熱安定性が変化し,タンパク質の折り畳みと機能を制御する方法を実証した.

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Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET12:07

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