関連する実験動画
Updated: Jul 21, 2026
11:19
Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
Published on: February 20, 2017
分裂酵母におけるサイクリン-cdc2の明確な核とスパインドルポール体群
C E Alfa1, B Ducommun, D Beach
1Department of Biology, University College London, UK.
Nature
|October 18, 1990
まとめ
この研究は,分裂酵母における2つの異なる核サイクリン群を明らかにし,一つはスパインドルの極点にある. これらのサイクリンは細胞サイクル進行を調節し,活性化にはマイクロチューブル機能が必要です.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- サイクリンは,真核細胞の細胞周期の重要な調節体であり,cdc2タンパク質キナーゼのサブユニットとして作用する.
- 分裂酵母Schizosaccharomyces pombeは,核に局所するcdc13遺伝子によってコードされたB型サイクリンを使用しています.
研究 の 目的:
- S. pombe. の核におけるcdc13サイクリンの空間的分布と機能を調査する.
- ミトスのスパインドル形成とcdc2の活性化におけるcdc2-サイクリン複合体の役割を明らかにする.
主な方法:
- S. pombe.におけるcdc13サイクリン局所化の顕微鏡分析.
- 抗微小管薬チアベンダゾールによる治療は,サイクリン分解とcdc2活性化に対する効果を評価する.
主要な成果:
- S. pombeの核で2つの異なるcdc13サイクリン群が特定されました.
- 1つのサイクリン群がミトスのスパインドルの極と関連していることが判明しました.
- 両方のサイクリン群は,cdc2遺伝子の産物であるp34cdc2とコロカライズした.
- シアベンダゾール治療はサイクリン分解を阻害し,cdc2活性化を阻害しました.
結論:
- cdc2-cyclin複合体は,ミトスのスパインドル形成を開始する上で重要な役割を果たします.
- 適切なミトーシス微小管の機能は,cdc2.の活性化に不可欠である.
関連する概念動画
02:37Restarting Stalled Replication Forks
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
01:29Cytoskeletal Proteins in Bacteria
Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
02:17Microtubule Instability
Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated assembly and...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
01:26Bacterial Protein Maturation
Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...