ELAM-1依存細胞の血管内皮への粘着は,感染したヒトフコシルトランスフェラーゼcDNAによって決定されます
J B Lowe1, L M Stoolman, R P Nair
1Howard Hughes Medical Institute, University of Michigan Medical School, Ann Arbor 48109-0650.
Cell
|November 2, 1990
まとめ
特定のフコシルトランスフェラーゼは,シアリルルイスx発現を制御することによって,白血球の内皮-白血球結合分子-1 (ELAM-1) に対する白血球結合を調節する. この発見は,炎症性細胞粘着における重要なメカニズムを明らかにしています.
科学分野:
- 免疫学 免疫学とは
- 分子生物学は分子生物学である.
- グライコバイオロジーは,
背景:
- 血管内皮への白血球の粘着は,炎症の間に極めて重要です.
- 内皮白血球粘着分子-1 (ELAM-1) は,その炭水化物認識領域 (CRD) を通してこのプロセスを媒介する.
- ELAM-1のCRDの正確な役割とその炭水化物結合特異性は,以前は不明でした.
研究 の 目的:
- ELAM-1のCRDが細胞粘着に与える貢献を調査する.
- ELAM-1の炭水化物結合特異性を決定する.
- ELAM-1-媒介による粘着におけるフコシルトランスフェラスの役割を解明する.
主な方法:
- ヒトのフコシルトランスフェラーゼcDNAを非ミエロイド細胞系に変異させる.
- ELAM-1-依存性内皮粘着の評価について.
- 炭水化物の決定因子の細胞表面表現を分析する.
主要な成果:
- トランスフェクトされた細胞は,ELAM-1に依存した内皮への粘着を示した.
- 粘着は,シアリル・ルイス×テトラサッカリドのデノボ表現と相関する.
- シアリルルイスxの生物合成は,感染したフコシルトランスフェラーゼ活性と直接関連していた.
結論:
- 特定のアルファ (1,3) フコシルトランスフェラーゼは,ELAM-1-媒介の細胞結合を調節する.
- これらの酵素は,シアリルルイスxおよび関連する構造の細胞表面表現を調節する.
- Sialyl Lewis xは,炎症中にELAM-1に結合する重要な決定因子です.
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