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関連する概念動画

Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze the...
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
Ladder Diagrams: Redox Equilibria01:30

Ladder Diagrams: Redox Equilibria

Ladder diagrams are useful tools for understanding redox equilibrium reactions, especially the effects of concentration changes on the electrochemical potential of the reaction. The vertical axis in the redox ladder diagrams represents the electrochemical potential, E. The area of predominance is demarcated using the Nernst equation.
Consider the Fe3+/Fe2+ half-reaction, which has a standard-state potential of +0.771 V. At potentials more positive than +0.771 V, Fe3+ predominates, whereas Fe2+...
Heterogeneous Catalysis01:22

Heterogeneous Catalysis

Heterogeneous catalysis involves a catalyst in a different phase from the reactants. It is a process where the catalyst and the reactants are in distinct phases, typically solid and gas or liquid.Most heterogeneous catalysts are metals, metal oxides, or acids. The list includes transition metals like iron (Fe), cobalt (Co), nickel (Ni), palladium (Pd), platinum (Pt), chromium (Cr), manganese (Mn), tungsten (W), silver (Ag), and copper (Cu). These metals possess partially vacant d orbitals that...
Electron Transport Chain Components01:29

Electron Transport Chain Components

The electron transport chain (ETC) is a crucial metabolic pathway that facilitates energy conversion in prokaryotic and eukaryotic cells. In eukaryotes, the ETC comprises four membrane-associated protein complexes in the inner mitochondrial membrane. In prokaryotes, the ETC in the plasma membrane can vary in composition, with fewer or different complexes depending on the organism and environmental conditions. These complexes transfer electrons from electron donors, such as NADH and FADH2, to...

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Design and Use of Multiplexed Chemostat Arrays
19:40

Design and Use of Multiplexed Chemostat Arrays

Published on: February 23, 2013

基板表示マトリックスのポリカタリティックアセンブリの振る舞い

Renjun Pei1, Steven K Taylor, Darko Stefanovic

  • 1NSF Chemical Bonding Center: Center for Molecular Cybernetics, Division of Experimental Therapeutics, Department of Medicine, Columbia University, New York, NY 10032, USA.

Journal of the American Chemical Society
|September 28, 2006
PubMed
まとめ

ニュークレア酸触媒 (デオキシリボ酵素) とストリープタヴィジンを使用して,新しいポリカタリティックアセンブリを作成しました. これらのアセンブリは,マトリックス上の基板を効率的に割って,潜在的なアプリケーションのための制御された動きと活動を提供します.

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Design, Surface Treatment, Cellular Plating, and Culturing of Modular Neuronal Networks Composed of Functionally Inter-connected Circuits
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In Vitro Multiparametric Cellular Analysis by Micro Organic Charge-modulated Field-effect Transistor Arrays
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In Vitro Multiparametric Cellular Analysis by Micro Organic Charge-modulated Field-effect Transistor Arrays

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Design and Use of Multiplexed Chemostat Arrays
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科学分野:

  • バイオケミストリー バイオケミストリー
  • 分子生物学は分子生物学である.
  • ナノテクノロジー ナノテクノロジー

背景:

  • 核酸触媒,またはデオキシリボ酵素は,プログラム可能な酵素活性を提供します.
  • 生物学的プロセスを模倣する効率的な触媒システムを開発することは,重要な課題です.

研究 の 目的:

  • フォスフォディエステラーゼ活性を持つポリカタリティックアセンブリを設計し,特徴づけること.
  • これらのアセンブリの制御された動きと触媒効率を基板に富んだマトリックス上で調査する.

主な方法:

  • 異なった数のデオキシリボ酵素とストレプタヴィジン単位を持つポリカタリティックアセンブリの構築.
  • 表面プラズモン共鳴 (SPR) を使用したアンサンブルレベルの特徴付け.
  • オリゴヌクレオチドコーティングマトリックスにおける触媒活性の観察.

主要な成果:

  • ポリカタリティックアセンブリは,高密度マトリックス上で連続した基板割れを示した.
  • 解離率は,溶液中の個々のデオキシリボジームと同等であった.
  • アセンブリの拡散,プロセシビティ,およびレジデント時間は,触媒ユニット数と認識シーケンスを変更することによって調整可能でした.

結論:

  • ポリカタリティックアセンブリは,制御された酵素活性のための新しいプラットフォームを表します.
  • 組み立てのダイナミクスを調整する能力は,洗練された分子機械のための可能性を開きます.
  • この研究は,エンジニアリングされた核酸触媒の分野を前進させています.