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宿主由来エピトープを標的とする非A,非B型肝炎特異抗体:自己免疫プロセスへの影響
Lancet (London, England)
|December 8, 1990
まとめ
研究者は,チンパンジーのプラズマからGORエピトープを特定し,それに対する抗体の測定法を開発しました. この検査は,非A型,非B型肝炎 (NANBH) の診断と,輸血後の肝炎の減少に役立ちます.
科学分野:
- ウイルス学 ウイルス学 ウイルス学
- 免疫学 免疫学とは
- 肝臓病理学 肝臓病理学
背景:
- 人間の非A,非B型肝炎 (NANBH) の診断は依然として困難です.
- C型肝炎ウイルス (HCV) はNANBHの主な原因ですが,すべての症例が既知のHCV配列によって説明されるわけではありません.
- 宿主細胞の反応は,NANBHの病原化に役割を果たす可能性があります.
研究 の 目的:
- NANBH剤に感染したチンパンジーのプラズマから特定された新しいエピトープ (GORエピトープ) を調査するために.
- GORエピトープ (anti-GOR) に対する抗体に対する酵素関連免疫吸収検査 (ELISA) の開発と検証.
- NANBHに対するアンチ-GORの診断的有用性と,輸血後の肝炎の軽減におけるその潜在的な役割を評価する.
主な方法:
- 感染したチンパンジーのプラズマからGORエピトープをコードするcDNAクローン (GOR47-1) の分離.
- 抗GOR抗体検出のための酵素関連免疫吸収測定法 (ELISA) の開発.
- 急性NANBH,慢性NANBH,NANB肝硬変,および原発性肝細胞癌を含む患者コホートの血清学的分析.
- 抗GORの流行と抗HCVの状態の比較と,ポリメラーゼ連鎖反応 (PCR) を使用したHCV-RNAの検出.
主要な成果:
- GORエピトープは宿主細胞配列によって暗号化され,報告されたHCV配列ではありませんでした.
- 抗GOR抗体 (IgMとIgG) は,急性NANBHの患者で検出されました.
- 慢性的なNANBH (81%),NANB肝硬変 (65%),NANB肝細胞癌 (63%) の患者で,抗GORの高い流行が観察されました.
- 抗GORは,他の肝疾患または健康なドナー (<10%) の患者でめったに検出されませんでした.
- HCV-RNAは,アンチ-HCVの陰性であったほとんどのGOR陽性患者で検出されました.
結論:
- GORエピトープは,潜在的にNANBH.と関連している宿主由来マーカーを表しています.
- 抗GOR ELISAは,特に抗HCV陰性症例において,NANBHを診断するための貴重なツールです.
- 抗GORの検出は,リスクのある個人を特定し,輸血後の肝炎を減らすのに役立ちます.
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