非自然なタンパク質融合を用いて,酵母と哺乳類の細胞におけるレスベラトロールのバイオシンセシスを設計する
Yansheng Zhang1, Song-Zhe Li, Jia Li
1Donald Danforth Plant Science Center, St. Louis, Missouri 63132, USA.
Journal of the American Chemical Society
|October 5, 2006
まとめ
研究者たちは,植物を設計した.
科学分野:
- バイオケミストリー バイオケミストリー
- 合成生物学 合成生物学とは
- メタボリックエンジニアリング
背景:
- 植物由来化合物であるレスベラトロールは,健康上の利点と寿命を延ばす特性を有しています.
- レスベラトロールのバイオシンセシスは,チロシンアンモニアライゼ (TAL),4-クウマレートCoA-リガゼ (4CL),スティルベネシンセチゼ (STS) などの特定の酵素を伴う.
研究 の 目的:
- 植物以外の宿主体において,新たなレスベラトロール生物合成経路を確立する.
- レスベラトロールの生産を強化する酵素局在化の効率を調査する.
主な方法:
- TAL,4CL,STSをコードする単離遺伝子は,異なる生物から採取したものです.
- 酵母とヒトのHEK293細胞を合成して,レスベラトロールを生産する.
- 酵素の近接性と経路の効率性を高めるために,翻訳的融合タンパク質を活用した.
主要な成果:
- 4CLとSTSを発現する酵母細胞は,4コマリック酸からレスベラトロールを産生した.
- 4CLとSTSのトランスレーション融合は,酵母におけるレスベラトロールの産生を15倍に増加させた.
- ヒトのHEK293細胞で,新たなレスベラトロールバイオシンセシスと細胞内蓄積が成功しました.
結論:
- 哺乳類の宿主における完全な植物天然産物経路を設計した.
- 酵素の物理的な局所化が代謝経路の効率を高めることを実証した.
- 哺乳類の細胞工場でレスベラトロールを生産するための可能性が開かれた.
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