ELFT:ELAM-1リガンドの発現を制御する遺伝子
Cell
|December 21, 1990
まとめ
研究者らは,ELAM-1リガンドにとって重要なフコシルトランスフェラーゼであるELFTを特定しました. この酵素は,
科学分野:
- 細胞の粘着性 細胞の粘着性
- 炎症生物学 炎症生物学とは
- 分子免疫学 分子免疫学
背景:
- 白血球内皮細胞粘着分子 (LECCAMs) は,炎症プロセスに関与しています.
- 内皮細胞粘着分子1 (ELAM-1) は,白血球が活性化された内皮細胞に粘着することを媒介する.
- ELAM-1の特定のリガンドは完全に特定されていません.
研究 の 目的:
- ELAM-1結合活性を与えるタンパク質をコードするcDNAをクローンする.
- ELAM-1リガンド認識の分子基盤を特定するために.
- 炎症調節におけるこの酵素の役割を調査する.
主な方法:
- ELFT (ELAM-1リガンドフコシルトランスフェラーゼ) と指定されたcDNAのクローニング.
- ELFTを非結合細胞系に変異させ,ELAM-1結合を評価する.
- 暗号化されたタンパク質の酵素活性と発現パターンの分析.
主要な成果:
- クローンされたcDNA,ELFTは,トランスフェクション時にELAM-1結合活性を与えます.
- ELFTは46 kdのタンパク質をコードし,アルファ ((1,3) フクソシルトランスフェラーゼ活性を持つ.
- ELFT発現は,ELAM-1に結合する細胞タイプに特異的です.
結論:
- フクソシル化炭水化物構造は,ELAM-1リガンドの重要な構成要素である可能性が高い.
- ELFTは,ELAM-1リガンドの合成における重要な酵素である.
- ELFTは,in vivoにおいて炎症的出来事を調節する上で重要な役割を果たします.
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