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関連する概念動画

Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Translational Regulation01:29

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...

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関連する実験動画

Updated: Jul 19, 2026

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

タンデムリボスイッチアーキテクチャは,複雑な遺伝子制御機能を示す.

Narasimhan Sudarsan1, Ming C Hammond, Kirsten F Block

  • 1Department of Molecular, Cellular and Developmental Biology, Yale University, Post Office Box 208103, New Haven, CT 06520-8103, USA.

Science (New York, N.Y.)
|October 14, 2006
PubMed
まとめ

バシルス・クラウジは,遺伝子発現を制御するユニークなタンデムリボスイッチシステムを備えています. このRNAベースのシステムは,S-アデノシルメチオニンとコエンザイムB12の2つの代謝物を統合し,タンパク質なしで複雑な遺伝的決定を下します.

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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

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Last Updated: Jul 19, 2026

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

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Published on: August 20, 2014

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

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科学分野:

  • 分子生物学は分子生物学である.
  • RNA 生物学 RNA 生物学
  • バクテリアの遺伝学

背景:

  • リボスイッチは,細菌の遺伝子発現を調節するRNA分子です.
  • 通常,リボスイッチは単一の代謝物を結合し,単純な遺伝子スイッチとして作用します.
  • mRNAの5'未翻訳領域に位置し,転写または翻訳を制御します.

研究 の 目的:

  • バシルス・クラウジーの遺伝子制御メカニズムを調査する. metE mRNA.
  • 新しいリボスイッチアーキテクチャとその規制機能を特定し,特徴づけること.
  • RNA要素が複雑な遺伝的論理ゲートを形成する方法を探求する.

主な方法:

  • バチルス・クラウジー・メテ mRNAのバイオ情報分析.
  • RNA構造の探査とメタボライト結合測定.
  • タンデムリボスイッチシステムの構築と特徴付け.

主要な成果:

  • Bacillus clausii metE mRNAの5'領域に2つの異なるリボスイッチを特定しました.
  • これらのリボスイッチは,S-アデノシルメチオニンとコエンザイムB12の両方に反応する.
  • タンデム配列は2つの入力ブール式NOR論理ゲート,複合遺伝子制御システムとして機能します.

結論:

  • 単純なRNA要素は,洗練された,マルチ入力遺伝的論理ゲートを形成することができます.
  • タンデムリボスイッチは,タンパク質に独立して複雑な遺伝子調節のためのメカニズムを提供します.
  • この発見は,バクテリアのRNAベースの規制ネットワークについての理解を広げています.