関連する実験動画

Updated: Jul 8, 2026

RhoC GTPase Activation Assay
09:58

RhoC GTPase Activation Assay

Published on: August 22, 2010

すべてのRho依存プロカリオット転写ターミネーターに共通するコンセンサスモチーフ

P Alifano1, F Rivellini, D Limauro

  • 1Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università di Napoli, Italy.

Cell
|February 8, 1991
PubMed
まとめ

研究者らは,サルモネラ・タイフィムリウム (Salmonella typhimurium) のオペロンにおける,暗号的なロア依存型転写終止要素を特定した. サイトシンが豊富でグアノシンが少ない特定のDNAモチーフは,これらのターミネーターに共通していることが判明しました.

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Transcription Initiation01:47

Transcription Initiation

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Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...