アクチン結合タンパク質テンシンにSH2ドメインが存在する
1Division of Cellular and Molecular Biology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115.
Science (New York, N.Y.)
|May 3, 1991
まとめ
研究者はテンシン断片をクローンし,Srcホモロジー2 (SH2) ドメインを明らかにした. このアクチン結合タンパク質は,フォスフォチロジンタンパク質と結合し,細胞シグナリングを細胞骨格と結びつけることを示唆しています.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- バイオケミストリー バイオケミストリー
背景:
- テンシンは,焦点結合と細胞骨格構造に含まれるアクチン結合タンパク質です.
- Srcホモロジー2 (SH2) ドメインは,信号伝導に不可欠であり,フォスフォチロジン結合を通じてタンパク質とタンパク質の相互作用を媒介する.
研究 の 目的:
- テンシン断片の分子クローニングを報告する.
- テンシンの機能領域,特に信号伝達における潜在的役割を調査する.
主な方法:
- 90キロダルトンのテンシン断片をコードする補完DNA (cDNA) の分子クローニング.
- アミノ酸配列の導出と分析.
- SH2ドメインのフォスフォチロジン結合活性を評価するための生化学的分析.
- インビトロリン酸化研究.
主要な成果:
- 90kDaのテンシン断片のcDNAをクローンに成功しました.
- 導出されたアミノ酸配列は,テンシンにおけるSrcホモロジー2 (SH2) ドメインの存在を確認した.
- テンシンSH2ドメインは,v-src-トランスフォーメーションされた細胞から,特にフォスフォチロシンを含むタンパク質に結合します.
- テンシンはチロシンキナーゼの基質として特定され,チロシン残基にリン酸化される.
結論:
- テンシンはアクチン結合能力とフォスフォチロジン結合能力の両方を備えています.
- テンシンのSH2ドメインは,信号分子との相互作用を媒介する役割を果たします.
- テンシンは,信号伝達経路と細胞骨格の間の潜在的な分子リンクとして作用します.
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