アンチセンセスの転写は,Saccharomyces cerevisiaeの細胞運命を制御する

Cintia F Hongay¹, Paula L Grisafi, Timothy Galitski

¹Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Cell

|November 18, 2006

まとめ

酵母へのメイオティック侵入は,IME4遺伝子のアンチセンセスのRNA制御によって制御されます. このメカニズムは,メオシスへの移行中に適切な細胞運命を決定することを保証します.

科学分野:

分子生物学は分子生物学である.
遺伝学遺伝学とは
細胞生物学細胞生物学

背景:

メイオシスの開始は,真核細胞における重要な発達過程である.
IME4遺伝子は,Saccharomyces cerevisiaeにおけるメオティック経路の開始に不可欠である.

研究の目的:

酵母菌に媒介性の侵入を制御する規制メカニズムを調査する.
IME4遺伝子発現の制御における反感覚媒介調節の役割を明らかにする.

主な方法:

異なった酵母菌株におけるIME4感覚と反感覚のトランスクリプトレベルの分析.
IME4転写に対するa1/alpha2ヘテロダイマー結合の影響を調査.
変化したIME4トランスクリプトレベルを持つ酵母菌株のフェノタイプ分析.

主要な成果:

IME4の反感覚調節は,Saccharomyces cerevisiaeの微分化の開始を制御する.
a1/alpha2ヘテロダイマーは,二倍体細胞におけるIME4アンチセンセスのトランスクリプトを抑制する.
IME4の感覚と反感覚のトランスクリプトの調節不良は,異質なメオティック開始と二分化細胞の二分化細胞のような現象型につながります.

結論:

IME4ロカスにおける反意味媒介による転写干渉は,細胞運命を決定する重要な規制メカニズムである.
この調節経路は,交配型シグナルに反応して,メオシスへの適切な進行を保証する.

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