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Updated: Jul 16, 2026

Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins
13:59

Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins

Published on: December 12, 2013

15N固体NMRは,酸化フラビン反応部位の感受性プローブを提供します

Ronald L Koder1, Joseph D Walsh, Maxim S Pometun

  • 1Department of Chemistry, University of Kentucky, Lexington, Kentucky 40506-0055, USA.

Journal of the American Chemical Society
|November 23, 2006
PubMed
まとめ

固体NMRは,フラビン窒素の反応性を明らかにします. この技術は,フラビン環の位置を直接観察し,電子構造とさまざまな酵素活動に関する洞察を提供します.

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¹H NMR of Labile Protons: Temporal Resolution01:10

¹H NMR of Labile Protons: Temporal Resolution

Protons bonded to heteroatoms such as nitrogen and oxygen exhibit a range of chemical shift values. This is due to the varying degree of hydrogen bonding between the proton and the heteroatom in other molecules. The extent of hydrogen bonding affects the electron density around the proton, thereby giving different chemical shift values for the protons in the proton NMR spectrum.
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¹H NMR of Labile Protons: Deuterium (²H) Substitution

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¹³C NMR: ¹H–¹³C Decoupling

The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
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