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関連する概念動画

Thermosensation01:43

Thermosensation

Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...
Multi-pass Transmembrane Proteins and β-barrels01:09

Multi-pass Transmembrane Proteins and β-barrels

In multi-pass transmembrane proteins, the polypeptide chain crosses the membrane more than once. The transmembrane polypeptide chain either forms an α-helix or β-strand structure. α-Helix containing multi-pass transmembrane proteins are ubiquitous, whereas β-strand containing ones are mainly found in gram-negative bacteria, mitochondria, and chloroplasts.
α-Helix containing multi-pass transmembrane proteins
Multi-pass transmembrane proteins such as G-protein-linked receptors (GPCRs) and...
Aquaporins01:25

Aquaporins

Aquaporins or AQPs are a family of integral membrane proteins whose primary function is to transport water, while some called aquaglyceroporins also transport glycerol. In addition, aquaporins have also been suspected to be involved in transporting volatile substances, such as carbon dioxide and ammonia, across membranes. Such AQPs that act as gas channels are often highly expressed in cells involved in the gaseous exchange, such as red blood cells, epithelial cells, and pulmonary capillaries.
Membrane Fluidity01:23

Membrane Fluidity

Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.Fatty acids tails of phospholipids can be either saturated or...
Membrane Fluidity01:26

Membrane Fluidity

Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is a relatively...
Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...

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関連する実験動画

Updated: Jul 18, 2026

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4
12:09

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

Published on: December 31, 2013

温度感受性タンパク質の毛穴は温度感受性タンパク質の毛穴である.

Yuni Jung1, Hagan Bayley, Liviu Movileanu

  • 1Department of Medical Biochemistry and Genetics, The Texas A&M University System Health Science Center, College Station, Texas 77843-1114, USA.

Journal of the American Chemical Society
|November 23, 2006
PubMed
まとめ

研究者らは,アルファ-ヘモリシン (alphaHL) 毛穴内のエラスティンのようなポリペプチド (ELP) ループを使用して,温度に反応するタンパク質の毛穴を設計した. これらの変形した毛穴は,温度制御された可逆のイオン流を示し,ELPの形状に基づいて開閉する.

科学分野:

  • バイオテクノロジー バイオテクノロジー
  • ナノテクノロジー ナノテクノロジー
  • プロテイン工学は,タンパク質の

背景:

  • アルファ-ヘモリシン (alphaHL) は,既知の結晶構造と大きな内腔を持つヘプトアメリカンタンパク質の孔である.
  • タンパク質の毛穴は生物学的システムにおいて極めて重要であり,感知および薬物投与における潜在的な応用がある.
  • 毛孔機能を動的に制御することは,高度な生体材料の開発における重要な課題です.

研究 の 目的:

  • エラスティンのようなポリペプチド (ELP) ループを組み込むことにより,温度に対応するタンパク質の毛穴を設計する.
  • アルファHL孔のイオン輸送特性に対するELPループ挿入の効果を調査する.
  • 医療バイオテクノロジーにおけるこれらの調節可能なタンパク質のポールの潜在的な応用を探求する.

主な方法:

  • アルファ-ヘモリシン (alphaHL) 孔の光腔に単一のエラスティン型ポリペプチド (ELP) ループを挿入する.
  • 応用電位下でのワイルド型およびELP改変アルファHL孔を通じたイオン輸送の特徴.
  • ELP移行温度と比較した異なる温度での毛孔の振る舞いの分析.

主要な成果:

  • 野生型アルファHLの毛穴は開いており,ELPを含む毛穴は一時的な電流の遮断を示しました.

さらに関連する動画

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice
08:35

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice

Published on: March 17, 2015

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

関連する実験動画

Last Updated: Jul 18, 2026

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4
12:09

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

Published on: December 31, 2013

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice
08:35

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice

Published on: March 17, 2015

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

  • ブロックの性質と頻度は,挿入されたELPループの長さと順番に依存していました.
  • ELPループは,移行温度より低い孔を可逆的に遮断し,脱水と崩壊により,その上のイオンフローを許しました.

    • 結論:

    • ELPループを備えた設計されたalphaHLの毛穴は,制御可能な,温度に依存するイオン輸送を現しています.
    • リバーシブルブロックメカニズムは,ELPの温度によって引き起こされる形状の変化に起因する.
    • これらの温度に反応するタンパク質の毛穴は,医療バイオテクノロジーやそれ以上の分野での応用が有望である.