関連する実験動画
Updated: May 11, 2026

08:39
Electroporation of Functional Bacterial Effectors into Mammalian Cells
Published on: January 19, 2015
タンパク質は,III型分泌装置によって,真核細胞に供給される
Jorge E Galán1, Hans Wolf-Watz
1Section of Microbial Pathogenesis, Yale University School of Medicine, Boyer Center for Molecular Medicine, New Haven, Connecticut 0636, USA. jorge.galan@yale.edu
Nature
|December 1, 2006
まとめ
バクテリアはIII型分泌システム (T3SS) を用いて,宿主細胞にタンパク質を注入する. この驚くべき適応は,様々な種の共生性および病原性細菌の生存と複製に不可欠です.
科学分野:
- 微生物学 微生物学とは
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
背景:
- ユカリオットと密接に暮らすバクテリアは,特殊な生存メカニズムを発達させる.
- 型III分泌システム (T3SS) は,宿主細胞と相互作用するための重要な細菌の適応である.
研究 の 目的:
- 細菌と真核生物の相互作用におけるIII型分泌システム (T3SS) の重要性を強調する.
- 様々な宿主-病原体,共生関係におけるT3SSの広範な影響を調査する.
主な方法:
- バクテリアの種間でT3SS遺伝子を特定するための比較ゲノミクス.
- ユーカリオット細胞へのタンパク質の供給を研究するための機能的測定法.
- T3SSの進化を理解するための系統遺伝分析.
主要な成果:
- T3SSは,病原体や共生生物を含む多様な細菌系統に存在する.
- このシステムは,エフェクタータンパク質を宿主細胞に直接注入するのに不可欠です.
- T3SSは,病原性および共生的な関連において重要な役割を果たします.
結論:
- タイプIII分泌システム (T3SS) は,細菌の宿主操作のための収束的な進化的適応を表しています.
- T3SSの研究は,臓器細胞の組み立て,タンパク質の分泌,共生,および病原性の基本的なプロセスについての洞察を提供します.
さらに関連する動画
関連する概念動画
Intracellular Movement of Viruses and Bacteria
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a virus that...
Overview of Secretory Vesicles
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Overview of Protein Sorting and Transport
Eukaryotic cells have different membrane-bound organelles with distinct protein requirements. The process by which proteins are targeted to a specific organelle is called protein sorting.
Protein sorting can be of two types: signal-based sorting and vesicle-based trafficking. In signal-based sorting, specific amino acid sequences called sorting signals target proteins to the proper location inside the cell either via gated transport or by protein translocation. In gated transport, folded...
Protein sorting can be of two types: signal-based sorting and vesicle-based trafficking. In signal-based sorting, specific amino acid sequences called sorting signals target proteins to the proper location inside the cell either via gated transport or by protein translocation. In gated transport, folded...
Insertion of Single-pass Transmembrane Proteins in the RER
Integral membrane proteins are proteins adhered to the lipid bilayer of a cell organelle or membrane. They can be of two types: transmembrane integral proteins that span the lipid bilayer and monotopic proteins that are attached to either side of the membrane but do not pass through it.
Integral transmembrane proteins possess transmembrane and extra membrane domains. The transmembrane domains are primarily made of 20-25 hydrophobic amino acids arranged in a helical secondary confirmation. These...
Integral transmembrane proteins possess transmembrane and extra membrane domains. The transmembrane domains are primarily made of 20-25 hydrophobic amino acids arranged in a helical secondary confirmation. These...
Bacterial Translocation and Protein Secretion
Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
Gram-negative Bacterial Protein Secretion Systems
Gram-negative bacteria utilize sophisticated protein secretion systems to transport proteins across their double-membrane envelope into the extracellular environment or host cells. Based on their mechanism of action, these systems are classified into one-step and two-step pathways.One-Step Secretion Systems (Types I, III, IV, and VI)One-step secretion systems bypass the periplasm entirely, forming a continuous channel that spans both the inner and outer membranes:Type I Secretion System (T1SS):...

