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Updated: Aug 4, 2026

Recombineering Homologous Recombination Constructs in Drosophila
14:23

Recombineering Homologous Recombination Constructs in Drosophila

Published on: July 13, 2013

P[acman]: D. melanogaster に大きなDNA断片をターゲットに挿入するためのBACトランスジェニックプラットフォーム

Koen J T Venken1, Yuchun He, Roger A Hoskins

  • 1Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA.

Science (New York, N.Y.)
|December 2, 2006
PubMed
まとめ

この研究では,Drosophila melanogasterの新型トランスジェネシスプラットフォームを導入しています. これは,遺伝子機能の研究のための効率的な大規模なDNA断片の統合を可能にし,果物ハエの遺伝学の研究を大きく前進させます.

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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...