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ヘアピンリボ酵素におけるG8の活性部位の標識:構造とメカニズムへの影響
Jason M Thomas1, David M Perrin
1Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, BC, Canada V6T 1Z1.
Journal of the American Chemical Society
|December 21, 2006
まとめ
研究者らは,ヘアピン・リボ酵素のグアノシン8 (G8) を,触媒の重要な成分として特定した. この発見は,アフィニティラベルを用いて,G8がRNase A.に類似して一般的なベースとして作用することを示唆しています.
科学分野:
- バイオケミストリー バイオケミストリー
- 分子生物学は分子生物学である.
- 酵素学 酵素学とは
背景:
- 一般的な酸塩触媒は,ヘアピンリボ酵素に対して提案されており,RNase A.に類似しています.
- リボ酵素における特定の触媒群と一般的な塩基触媒の範囲について議論されています.
研究 の 目的:
- ヘアピン・リボ酵素における一般的な塩基触媒の化学群を特定する.
- RNase Aに用いられる技術である親和性ラベリングをリボ酵素に適用する.
主な方法:
- 2'-ブロモアセタミド群との親和性ラベルを合成した.
- サブストラット割れ部位のヘアピンリボ酵素に親和感ラベルを貼った.
主要な成果:
- ヘアピンリボ酵素の特にアルキル化されたグアナシン8 (G8) は,おそらくN1位置にある.
- このアルキル化により,リボ酵素の活性部位化学におけるG8のN1が関与する.
結論:
- 発見は,G8のN1がヘアピンリボ酵素触媒の一般的ベースとして作用し,RNase A.を反映するという仮説を裏付けている.
- G8による水媒介触媒または移行状態の安定化を含む代替メカニズムも妥当である.
関連する概念動画
01:23RNA Structure
Overview
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Different Types of RNA Have the Same Basic Structure
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The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
01:23RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
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The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
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02:47Ribozymes
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Ribozymes can be...
02:47Ribozymes
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
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01:19RNA Structure
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
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There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...