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関連する概念動画

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
¹H NMR Signal Integration: Overview00:58

¹H NMR Signal Integration: Overview

The intensity of a signal, which can be represented by the area under the peak, depends on the number of protons contributing to that signal. The area under each peak is shown as a vertical line called an integral, with the integral value listed under it, as seen in the proton NMR spectrum of benzyl acetate. Each integral value is divided by the smallest integral value to obtain the ratio of the number of protons producing each signal. The ratio reveals the relative number of protons and not...
¹H NMR Signal Multiplicity: Splitting Patterns01:13

¹H NMR Signal Multiplicity: Splitting Patterns

When protons A and X are coupled, their nuclear spin energy levels are slightly modified. This is because the energy required to excite proton A to a spin state parallel to proton X is slightly different from the energy required for it to become anti-parallel to spin X. Consequently, there are two possible excitation frequencies for A (A1 and A2), depending on the spin state of X, and vice versa. The mutual nature of coupling implies that the difference between frequencies A1 and A2, indicated...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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関連する実験動画

Updated: Jul 14, 2026

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

多価インタフェースにおける超分子複合体の表現

Olga Crespo-Biel1, Choon Woo Lim, Bart Jan Ravoo

  • 1Laboratories for Supramolecular Chemistry & Technology and Molecular Nanofabrication, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.

Journal of the American Chemical Society
|December 21, 2006
PubMed
まとめ

研究者は,サイクロデクストリン (CD) ホスト・ゲストおよび金属イオン調整を用いて多価結合を調査した. 表面での結合強化を大幅に達成し,高度な材料に対する強力な超分子戦略を示した.

さらに関連する動画

Covalent Labeling with Diethylpyrocarbonate for Studying Protein Higher-Order Structure by Mass Spectrometry
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Covalent Labeling with Diethylpyrocarbonate for Studying Protein Higher-Order Structure by Mass Spectrometry

Published on: June 15, 2021

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

関連する実験動画

Last Updated: Jul 14, 2026

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

Covalent Labeling with Diethylpyrocarbonate for Studying Protein Higher-Order Structure by Mass Spectrometry
10:36

Covalent Labeling with Diethylpyrocarbonate for Studying Protein Higher-Order Structure by Mass Spectrometry

Published on: June 15, 2021

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

科学分野:

  • 超分子化学とは
  • 表面化学について
  • 協調化化学について

背景:

  • マルチバレント相互作用は結合親和性を高めます.
  • オートゴーナルホスト・ゲストと協調化学は,多用途の分子組み立てツールを提供します.
  • 表面ベースのアッセイは,インターフェースでの結合現象を研究するために不可欠です.

研究 の 目的:

  • 超分子複合体の多価結合を多価宿主表面に記述する.
  • オートゴーナルベータ・サイクロデクストリン (CD) ホスト・ゲストと金属イオン・エチレン・ダイアミン・コーディネーションモチーフを組み合わせる.
  • ヘテロトロプ的多価結合モデルを使用して,インターフェースでの結合強化を定量化します.

主な方法:

  • 二価結合剤として,アダマンチル-機能化されたエチレンダイアミン誘導体を利用した.
  • Cu (II) または Ni (II) メタルイオンを含む複雑なリンク器.
  • 表面プラズモン共振 (SPR) スペクトルスコピーを用いて,pHの関数として,CDの自己組み立てモノレイヤー (SAM) に結合することを研究した.

主要な成果:

  • ヘテロトロプ的結合モデルを用いた表面での量化された多価増強.
  • 溶液と比較して,CD表面上のCu (II) 複合体の結合強化因数>100が観察されました.
  • CD SAMのCu (II) システムとNi (II) システムの双価結合が確認されましたが,Ni (II) ケースでは三価結合の可能性があります.

結論:

  • 多価表面結合のためのCDホスト-ゲストと金属の協調の成功した組み合わせが実証されました.
  • 超分子複合化により,表面に重要な結合強化を達成した.
  • SPRの有用性と,インターフェイス相互作用を特徴付けるための多価結合モデルを検証した.