YY1は,インビトロトランスクリプションを誘導し,活性化するイニシアター配列結合タンパク質です

E Seto¹, Y Shi, T Shenk

¹Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, New Jersey 08544-1014.

Nature

|November 21, 1991

まとめ

転写因子YY1は,アデノ関連ウイルス2型P5プロモーターの+1領域に結合し,転写の開始を調節する. この結合は基礎転写に不可欠であり,他の遺伝子のYY1関連因子を含む.

科学分野:

分子生物学は分子生物学である.
遺伝子規制遺伝子規制
バイオケミストリーバイオケミストリー

背景:

ユーカリオットのmRNAの転写は,DNA配列要素と転写因子によって調節されます.
鍵となる要素には,プロモーター近接領域,強化器,抑制器,静音器が含まれています.
TATA結合因子はよく研究されているが,+1開始領域の因子はあまり理解されていない.

研究の目的:

転写におけるアデノ関連ウイルス2型P5プロモーターの+1領域の役割を調査する.
P5+1要素と相互作用する転写因子を特定する.
基礎転写のためのP5 +1要素の必要性と十分性を決定する.

主な方法:

DNA結合因子を特定するために,電泳運動移転シフトアッセイ (EMSA) を用いる.
ヘラとドロソフィラの抽出物を用いた枯渇と再構成の実験.
転写因子YY1 (YY1) とYY1特異性抗体を用いた機能分析.

主要な成果:

P5+1要素は,転写因子YY1.1.と結合する.
この要素は,正確な基礎転写に必要かつ十分である.
部分的に浄化されたYY1は,YY1欠乏した抽出物における転写を回復させ,YY1抗体は再活性化を阻害した.
他の細胞遺伝子の転写イニシアターと結合するYY1に関連する要因が特定されました.

結論:

転写因子YY1は,P5+1要素での転写開始を調節する上で重要な役割を果たします.
P5+1要素は,基礎転写の重要な規制領域である.
YY1および関連する要因は,さまざまな遺伝子にわたる転写の開始に関与しています.

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