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Updated: Jul 17, 2026

Rapid Determination of Antibody-Antigen Affinity by Mass Photometry
10:17

Rapid Determination of Antibody-Antigen Affinity by Mass Photometry

Published on: February 8, 2021

抗体-炭水化物-抗原複合体の相対結合エネルギーは,自由エネルギーシミュレーションから計算されたものです

A Pathiaseril1, R J Woods

  • 1Complex Carbohydrate Research Center, The University of Georgia, Athens, Georgia 30602, USA.

Journal of the American Chemical Society
|January 11, 2007
PubMed
まとめ

自由エネルギー干渉 (FEP) シミュレーションは,サルモネラ三糖化類の類似体と抗体断片の結合エネルギーを正確に予測しました. この計算方法は,炭水化物を結合するタンパク質のための高親和性リガンドを設計するのに役立ちます.

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Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms15:27

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

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関連する概念動画

The Equilibrium Binding Constant and Binding Strength02:18

The Equilibrium Binding Constant and Binding Strength

The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
The Equilibrium Binding Constant and Binding Strength02:18

The Equilibrium Binding Constant and Binding Strength

The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
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Affinity and Avidity

Overview
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Conserved Binding Sites

Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
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Noncovalent Attractions in Biomolecules

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Four types of noncovalent interactions are hydrogen bonds, van der Waals forces, ionic bonds, and hydrophobic interactions.
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Antibody Actions

Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
Neutralization
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