Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Ribozymes02:47

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Ribozymes02:47

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Diels–Alder vs Retro-Diels–Alder Reaction: Thermodynamic Factors01:31

Diels–Alder vs Retro-Diels–Alder Reaction: Thermodynamic Factors

The Diels–Alder reaction is thermally reversible, meaning that the reaction reverts to the starting diene and dienophile under suitable temperatures. The forward reaction gives a cyclohexene derivative and is favored at low to medium temperatures. The reverse process, also called retro-Diels–Alder reaction, is a ring-opening process favored at high temperatures.
Diels–Alder Reaction Forming Cyclic Products: Stereochemistry01:28

Diels–Alder Reaction Forming Cyclic Products: Stereochemistry

The Diels–Alder reaction is one of the robust methods for synthesizing unsaturated six-membered rings. The reaction involves a concerted cyclic movement of six π electrons: four π electrons from the diene and two π electrons from the dienophile.
Diels–Alder Reaction: Characteristics of Dienes01:29

Diels–Alder Reaction: Characteristics of Dienes

The Diels–Alder reaction brings together a diene and a dienophile to form a six-membered ring. Both components have unique characteristics that influence the rate of the reaction.
Characteristics of the diene
Conformation
The simplest example of a diene is 1,3-butadiene, an acyclic conjugated π system. At room temperature, the molecule exists as a mixture of s-cis and s-trans conformers by virtue of rotation around the carbon–carbon single bond. Although the s-trans isomer is more stable, the...
Diels–Alder Reaction: Characteristics of Dienophiles01:24

Diels–Alder Reaction: Characteristics of Dienophiles

In a Diels–Alder reaction, the diene is usually an electron-rich system and acts as a nucleophile, whereas the dienophile is electron-deficient and functions as an electrophile. Much like the diene, the nature of the dienophile significantly impacts the outcome of the reaction.
Characteristics of Dienophiles
Generally, the best dienophiles are alkenes containing electron-withdrawing substituents such as carbonyl, nitrile, and nitro groups. The feasibility of a Diels–Alder reaction depends on...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Proto-oncogene RTL4 promotes tumorigenesis and invasiveness of papillary thyroid cancer.

American journal of translational research·2020
Same author

Downregulated CDH3 decreases proliferation, migration, and invasion in thyroid cancer.

American journal of translational research·2020
Same author

Inhibition of C3a/C3aR Axis in Diverse Stages of Ulcerative Colitis Affected the Prognosis of UC by Modulating the Pyroptosis and Expression of Caspase-11.

Inflammation·2020
Same author

Hypertensive Retinopathy Secondary to Anlotinib Treatment.

Frontiers in pharmacology·2020
Same author

Sensitive photoelectrochemical assay of Pb<sup>2+</sup> based on DNAzyme-induced disassembly of the "Z-scheme" TiO<sub>2</sub>/Au/CdS QDs system.

Chemical communications (Cambridge, England)·2020
Same author

Engineered macroporous hydrogel scaffolds via pickering emulsions stabilized by MgO nanoparticles promote bone regeneration.

Journal of materials chemistry. B·2020

関連する実験動画

Updated: Jul 17, 2026

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
19:16

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis

Published on: March 17, 2010

ディエルス・アルダーリボ酵素触媒: 計算によるアプローチ

Xiaohua Zhang1, Thomas C Bruice

  • 1Department of Chemistry and Biochemistry, University of California, Santa Barbara, California 93106, USA.

Journal of the American Chemical Society
|January 25, 2007
PubMed
まとめ

この研究では,ダイエルス-アルデラーゼリボエンザイムと水の反応を計算的に比較しています. リボ酵素の活性部位は,反応物質を最適な位置と角度で結合させ,水と比較して触媒を強化します.

科学分野:

  • バイオケミストリー バイオケミストリー
  • コンピューティング・ケミストリー
  • 酵素学 酵素学とは

背景:

  • ディエルス・アルダー反応は,重要な炭素-炭素結合形成反応である.
  • リボ酵素,RNA酵素は,ディエルス・アルダー反応を含む様々な化学変化を触媒化することができます.
  • 酵素活性部位のメカニズムを理解することは,効率的な触媒の設計の鍵です.

研究 の 目的:

  • ディエルス-アルデラーゼリボ酵素によって触媒化されたディエルス-アルデラーゼ反応機構と,水中の触媒化されていない反応を計算的に比較する.
  • リボエンザイム活性部位の構造とダイナミクスの作用をカタリシスで明らかにする.
  • ディエルス-アルデラーゼリボエンザイムによる速度増強の起源を調査する.

主な方法:

  • SCCDFTB/MM (Self-Consistent Charge Density Functional Tight Binding/Molecular Mechanics) を用いたコンピューティング・モデリングを行いました. SCCDFTB/MM (Self-Consistent Charge Density Functional Tight Binding/Molecular Mechanics) を用いたコンピューティング・モデリングを行いました. SCCDFTB/MM (Self-Consistent Charge Density Functional Tight Binding/Molecular Mechanics) を用いたコンピューティング・モデリングを行いました. SCCDFTB/MM (Self-Consistent Charge Density Functional Tight Binding/Molecular Mechanics) を用いたコンピュータ・モデリングを行いました.

さらに関連する動画

Multiscale Sampling of a Heterogeneous Water/Metal Catalyst Interface using Density Functional Theory and Force-Field Molecular Dynamics
10:52

Multiscale Sampling of a Heterogeneous Water/Metal Catalyst Interface using Density Functional Theory and Force-Field Molecular Dynamics

Published on: April 12, 2019

Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation
15:05

Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation

Published on: May 20, 2020

関連する実験動画

Last Updated: Jul 17, 2026

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
19:16

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis

Published on: March 17, 2010

Multiscale Sampling of a Heterogeneous Water/Metal Catalyst Interface using Density Functional Theory and Force-Field Molecular Dynamics
10:52

Multiscale Sampling of a Heterogeneous Water/Metal Catalyst Interface using Density Functional Theory and Force-Field Molecular Dynamics

Published on: April 12, 2019

Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation
15:05

Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation

Published on: May 20, 2020

  • 自由エネルギーの障壁を計算するための傘のサンプリング技術.
  • 反応座標,移行状態,原子電荷の分析.

    • 主要な成果:

    • リボ酵素活性部位は反応物質を好ましい形状に保持し,アントラセンの場合は特定の傾斜アプローチ角度を持つ.
    • アクティブサイトは,移行状態よりも製品をより安定させる.
    • リボエンザイム触媒反応の計算された自由エネルギーバリアは,実験値と密接に一致します.
    • アクティブサイトダイナミクスは,触媒に最小限に貢献することが判明しました.

    結論:

    • ディエルス-アルデラーゼリボエンザイムは,活性部位における反応物質を事前組織化することによって,反応速度を高めます.
    • 活性部位の特定の幾何学と反応物質の位置は,触媒の熟練度にとって重要である.
    • 計算方法は,リボ酵素触媒によるディエルス・アルダー反応の実験観察を正確に再現します.